Stevens S E, Nierzwicki-Bauer S A, Balkwill D L
J Bacteriol. 1985 Mar;161(3):1215-8. doi: 10.1128/jb.161.3.1215-1218.1985.
The effects of nitrogen starvation on the morphology and ultrastructure of the branching, filamentous cyanobacterium Mastigocladus laminosus were examined with light and electron microscopy. The internal ultrastructural characteristics of vegetative cells changed markedly during nitrogen starvation. Carboxysomes were degraded, while polyphosphate bodies and lipid bodies accumulated. The ultrastructure of mature heterocysts was also affected by nitrogen starvation; their intracytoplasmic membranes vesiculated to form vacuolelike structures and, eventually, large empty regions in the cytoplasm. Nitrogen starvation stimulated extensive heterocyst differentiation in M. laminosus, producing heterocyst frequencies of 17.5% in narrow filaments and 28.3% in wide filaments within 44 h after transfer to N-free conditions. Cells in wide filaments differentiated so extensively that only 16.8% of them failed to initiate the differentiation process within 44 h.
利用光学显微镜和电子显微镜研究了氮饥饿对分支丝状蓝细菌层理鞭枝藻的形态和超微结构的影响。在氮饥饿期间，营养细胞的内部超微结构特征发生了显著变化。羧酶体降解，而多聚磷酸盐体和脂质体积累。成熟异形胞的超微结构也受到氮饥饿的影响；它们的胞内膜形成囊泡状结构，最终在细胞质中形成大的空区。氮饥饿刺激了层理鞭枝藻中广泛的异形胞分化，在转移到无氮条件后44小时内，窄丝中的异形胞频率为17.5%，宽丝中的异形胞频率为28.3%。宽丝中的细胞分化非常广泛，以至于在44小时内只有16.8%的细胞没有启动分化过程。
