Nierzwicki-Bauer S A, Balkwill D L, Stevens S E
Stain Technol. 1984 May;59(3):163-70. doi: 10.3109/10520298409113850.
A new light microscopic method for identifying heterocysts and proheterocysts in morphologically complex cyanobacteria was evaluated for reliability and usefulness. Mature heterocysts and proheterocysts could be distinguished readily from vegetative cells in 0.25 micron sections of fixed and embedded material after staining with toluidine blue. Examination by light and electron microscopy of the same specimens indicated that the staining reactions which served to differentiate these cell types were both reproducible and accurate. Light microscopic analysis of serial sections stained with toluidine blue greatly facilitated localization of heterocysts and proheterocysts in the complex, branching cyanobacterium, Mastigocladus laminosus, even when its filaments of cells were intertwined in thick mats.
对一种用于识别形态复杂的蓝细菌中异形胞和原异形胞的新型光学显微镜方法的可靠性和实用性进行了评估。在用甲苯胺蓝染色后，在固定和包埋材料的0.25微米切片中，成熟的异形胞和原异形胞可以很容易地与营养细胞区分开来。对相同标本进行光学显微镜和电子显微镜检查表明，用于区分这些细胞类型的染色反应是可重复且准确的。对用甲苯胺蓝染色的连续切片进行光学显微镜分析，极大地促进了在复杂的分支蓝细菌层理鞭枝藻中异形胞和原异形胞的定位，即使其细胞丝在厚厚的垫子中相互缠绕。
