Analytical & Testing Center, Sichuan University, Chengdu 610064, Sichuan, China.
Department of Rheumatology and Immunology, West China Hospital, Sichuan University, Chengdu 610041, Sichuan, China.
Anal Chem. 2024 Sep 10;96(36):14679-14687. doi: 10.1021/acs.analchem.4c03617. Epub 2024 Aug 27.
Urinary tract infection (UTI) is a common and prevalent disease caused by a spectrum of pathogens. Lack of access to rapid, portable, and high-quality diagnostics in resource-limited settings aggravates the improper treatment of UTIs, which is also a major driver of antibiotic misuse worldwide. Here, we describe a custom-made portable colorimetric array (PoCA) for reading out polymerase chain reaction (PCR) amplicons, the rationale of which is to transfer the previously developed dsDNA-based photosensitization colorimetric assay (solution) onto paper discs for detection. By integrating mini-LED irradiation and paper discs, the PoCA can read out 96 PCR tests in one pot, thus allowing diagnosis and identification of 12 prevailing UTI pathogens in less than 2 h, coupled with a portable thermal cycler for PCR. After analyzing 200 clinical urine samples, the pathogen profiling accuracy of the PoCA was demonstrated to be higher than the standard urine culture (confirmed with metagenomic next-generation sequencing). The PoCA platform could be used in primary care for rapid UTI diagnosis and pathogen identification.
尿路感染（UTI）是一种由多种病原体引起的常见且普遍的疾病。在资源有限的环境中，缺乏快速、便携和高质量的诊断方法，加剧了 UTI 的不当治疗，这也是全球抗生素滥用的主要驱动因素。在这里，我们描述了一种用于读取聚合酶链反应（PCR）扩增子的定制便携式比色阵列（PoCA），其原理是将先前开发的基于双链 DNA 的光致敏比色测定法（溶液）转移到纸盘上进行检测。通过整合 mini-LED 照射和纸盘，PoCA 可以在一个锅中读取 96 个 PCR 测试，从而可以在不到 2 小时的时间内诊断和识别 12 种常见的 UTI 病原体，并结合用于 PCR 的便携式热循环仪。在分析了 200 份临床尿液样本后，证明 PoCA 的病原体分析准确性高于标准尿液培养（经宏基因组下一代测序证实）。PoCA 平台可用于初级保健，以快速诊断 UTI 和鉴定病原体。
