College of Chemistry and Chemical Engineering, Northwest Normal University, Lanzhou, 730070, Gansu, PR China.
Anal Bioanal Chem. 2024 Nov;416(26):5841-5851. doi: 10.1007/s00216-024-05511-6. Epub 2024 Aug 27.
In this study, a ratiometric fluorescent sensor CdTe QDs@ZIF-8 with butterfly spectra is successfully constructed by in situ encapsulating mercaptopropionic acid-modified CdTe quantum dots in zeolitic imidazolate framework-8 (ZIF-8) with a simple strategy, and used for the detection of tetracycline in fluorescence/smartphone colorimetry dual-mode. ZIF-8 not only reduces the agglomeration of the quantum dots but also surprisingly generates a new green fluorescence signal at 524 nm while the red fluorescence of the CdTe quantum dots at 650 nm quenches when tetracycline is added. The two opposing fluorescence signals create a butterfly-shaped fluorescence spectrum, allowing the sensor to detect tetracycline over a linear range of 0-70 μM with the detection limit (LOD) of 0.0155 μM by using a ratiometric fluorescence technique. What is more, based on the obvious color change of the fluorescent sensor gradually from red to green under UV light, a highly stable point-of-care testing sensor has been developed for on-site detection of tetracycline through color recognition by smartphones, which can be used for real-time detection of this antibiotic in the range of 0-1000 μM with the LOD of 0.0249 μM. This work provides a simple and efficient method for the on-site detection of tetracycline.
在这项研究中，通过一种简单的策略，成功构建了一种具有蝶形光谱的比率荧光传感器 CdTe QDs@ZIF-8，该传感器通过原位封装巯基丙酸修饰的 CdTe 量子点在沸石咪唑酯骨架-8（ZIF-8）中，用于荧光/智能手机比色法双模式下四环素的检测。ZIF-8 不仅减少了量子点的聚集，而且当加入四环素时，令人惊讶地在 524nm 处产生了新的绿色荧光信号，而 CdTe 量子点的红色荧光在 650nm 处猝灭。这两个相反的荧光信号产生了蝴蝶形的荧光光谱，使得传感器能够通过比率荧光技术检测 0-70μM 范围内的四环素，检测限（LOD）为 0.0155μM。更重要的是，基于荧光传感器在紫外光下逐渐从红色变为绿色的明显颜色变化，通过智能手机的颜色识别，开发了一种高度稳定的即时检测传感器，用于现场检测四环素，可用于实时检测抗生素的范围在 0-1000μM 之间，LOD 为 0.0249μM。这项工作为四环素的现场检测提供了一种简单有效的方法。
