School of Optometry and Vision Science, University of New South Wales Sydney, Sydney, New South Wales, Australia.
Optom Vis Sci. 2024 Sep 1;101(9):603-607. doi: 10.1097/OPX.0000000000002172. Epub 2024 Aug 28.
This is the first study to evaluate the retention time of lubricating eye drops containing various concentrations of sodium hyaluronate using fluorophotometry in a symptomatic dry eye population. Information regarding eye drop retention may be useful for eye care practitioners to assist in the selection of more effective treatments for managing dry eye.
This study aimed to use fluorophotometry to compare retention time on the ocular surface of three commercially available lubricating eye drops, each containing varying concentrations of sodium hyaluronic acid (HA), and their effects on tear film stability post-instillation in a population with symptoms of dry eye.
Adults with symptoms of dry eye (Ocular Surface Disease Index score, >12) were enrolled in this prospective, double-masked comparison of eye drops containing 0.15% HA-hydroxypropyl guar (HPGuar), 0.2% HA, and 0.1% HA. Participants were randomized to the eye drop order and the study eye under evaluation. Each eye drop was admixed with a fluorescent tracer (70-kDa fluorescein isothiocyanate-dextran) at 10% wt/vol, and 10 μL volume was instilled for each evaluation. A fluorophotometer was used to measure the time for the tracer signal to return to baseline. Fluorescein tear breakup time was measured following fluorophotometry assessment.
Retention time for 0.15% HA-HPGuar and 0.2% HA was significantly longer compared with 0.1% HA (p=0.02 and p=0.03). Fluorescein tear breakup time was significantly longer for the 0.15% HA-HPGuar eye drop compared with both the 0.1% HA eye drop (p=0.01) and 0.2% HA eye drop (p=0.003).
Retention time on the ocular surface of the two eye drops containing higher concentrations of HA was longer than the eye drop with the lowest concentration of HA. The tear film was also more stable with the 0.15% HA-HPGuar eye drop compared with the eye drops containing HA alone, which may be attributable to the other components in the 0.15% HA-HPGuar eye drop.
这是第一项使用荧光光度法评估含有不同浓度透明质酸钠的润滑滴眼液在有症状的干眼人群中的保留时间的研究。关于滴眼液保留时间的信息可能对眼保健从业者有用，以帮助选择更有效的治疗方法来管理干眼症。
本研究旨在使用荧光光度法比较三种市售的含有不同浓度透明质酸钠（HA）的润滑滴眼液在有干眼症状的人群中的眼表面保留时间及其滴注后对泪膜稳定性的影响。
患有干眼症状（眼表面疾病指数评分>12）的成年人被纳入这项前瞻性、双盲对照研究，比较含有 0.15%HA-羟丙基瓜尔（HPGuar）、0.2%HA 和 0.1%HA 的滴眼液。参与者按照滴眼液顺序和评估眼随机分组。每滴滴眼液均与荧光示踪剂（70kDa 异硫氰酸荧光素-葡聚糖）以 10%wt/vol 混合，每次评估滴入 10μL 体积。使用荧光光度计测量示踪信号返回基线的时间。在荧光光度法评估后测量荧光素泪膜破裂时间。
0.15%HA-HPGuar 和 0.2%HA 的保留时间明显长于 0.1%HA（p=0.02 和 p=0.03）。0.15%HA-HPGuar 滴眼液的荧光素泪膜破裂时间明显长于 0.1%HA 滴眼液（p=0.01）和 0.2%HA 滴眼液（p=0.003）。
两种含有较高浓度 HA 的滴眼液在眼表面的保留时间长于含有最低浓度 HA 的滴眼液。与单独含有 HA 的滴眼液相比，0.15%HA-HPGuar 滴眼液的泪膜也更稳定，这可能归因于 0.15%HA-HPGuar 滴眼液中的其他成分。
