Li Meilin, Bao Yixue, Chen Wenhan, Li Yisha, Du Jinxia, Khan Abdullah, Khan Muhammad Tahir, Powell Charles A, Chen Baoshan, Zhang Muqing
Guangxi Key Laboratory for Sugarcane Biology & State Key Laboratory of Conservation and Utilization for Subtropical Agri-Biological Resources, Guangxi University, Nanning, Guangxi, 530005, China.
National Institute for Biotechnology and Genetic Engineering (NIBGE), Faisalabad, Pakistan.
Phytopathology. 2025 Jan;115(1):20-34. doi: 10.1094/PHYTO-03-24-0076-R. Epub 2025 Jan 13.
spp. are plant pathogens known for significantly impacting crop yields. Among them, () is notable for colonizing the xylem and causing sugarcane leaf scald disease. This study employed homologous recombination to mutate quorum sensing regulatory genes () to investigate their role in pathogenicity. Deletions of (Δ), (Δ), and (Δ) led to reduced swarming, growth, and virulence. However, diffusible signal factor (DSF) supplementation restored swarming and growth in the Δ mutant. Deleting , , and also reduced twitching motility and affected type IV pilus expression. Transcriptomic analysis revealed that Δ positively regulates flagellar genes. DSF supplementation in Δ (Δ-DSF) modulated the expression of flagellar, chemotaxis, and type IV pilus genes. These findings elucidate the DSF-mediated swarming pathway in and provide valuable insights into its regulatory mechanisms.
某些物种是已知对作物产量有重大影响的植物病原体。其中，（某物种）因定殖于木质部并引起甘蔗叶焦病而引人注目。本研究采用同源重组对群体感应调控基因（某些基因）进行突变，以研究它们在致病性中的作用。（某些基因）的缺失（Δ某基因）导致群体运动、生长和毒力降低。然而，补充可扩散信号因子（DSF）可恢复Δ突变体中的群体运动和生长。删除（某些基因）也会降低颤动运动性并影响IV型菌毛表达。转录组分析表明，Δ某基因正向调节鞭毛基因。在Δ某基因（Δ某基因-DSF）中补充DSF可调节鞭毛、趋化性和IV型菌毛基因的表达。这些发现阐明了DSF介导的（某物种）群体运动途径，并为其调控机制提供了有价值的见解。
