Laboratório de Patologia Animal, Universidade do Estado de Santa Catarina, Lages, Santa Catarina, Brazil.
Centro de Controle de Zoonoses, Lages, Santa Catarina, Brazil.
J Comp Pathol. 2024 Oct;214:12-18. doi: 10.1016/j.jcpa.2024.07.007. Epub 2024 Aug 26.
Feline leukemia virus (FeLV) is a highly debilitating cat pathogen due to its ability to cause many pathological changes. Therefore, identifying the virus directly in bone marrow can be a highly relevant diagnostic tool even in the absence of viraemia. The aim of this study was to compare the diagnostic efficiency of immunocytochemistry (ICC) of bone marrow aspirates with enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR). Blood samples were collected from 188 cats and separated into aliquots of whole blood for nested PCR using the U3 LTR region and the gag gene of FeLV-A as reference and serum for detection of the p27 antigen by ELISA. Bone marrow samples from these cats were placed on silanized slides for anti-FeLV ICC using gp70 as primary antibody. A total of 28.2% of the cats tested for FeLV were positive in at least one of the tests, with 26.6% positive by PCR, 18.1% by ICC and 11.2% by ELISA. Cohen's kappa agreement test revealed moderate agreement between ELISA and PCR results and substantial agreement between ICC and ELISA and between ICC and PCR. The results indicated that ICC of bone marrow is an efficient novel diagnostic test for FeLV infection.
猫白血病病毒（FeLV）是一种高度削弱猫的病原体，因为它能够引起许多病理变化。因此，即使没有病毒血症，直接在骨髓中检测病毒也可以成为一种非常相关的诊断工具。本研究的目的是比较骨髓抽吸物免疫细胞化学（ICC）与酶联免疫吸附试验（ELISA）和聚合酶链反应（PCR）的诊断效率。从 188 只猫中采集血液样本，并将其分成全血等分试样，用于使用 U3 LTR 区域和 FeLV-A 的 gag 基因进行巢式 PCR，作为参考，以及用于通过 ELISA 检测 p27 抗原的血清。从这些猫中采集骨髓样本，用于使用 gp70 作为主要抗体进行抗 FeLV ICC。在至少一项测试中，有 28.2%的猫检测到 FeLV 呈阳性，其中 26.6%通过 PCR 检测呈阳性，18.1%通过 ICC 检测呈阳性，11.2%通过 ELISA 检测呈阳性。Cohen's kappa 一致性检验显示，ELISA 和 PCR 结果之间存在中度一致性，ICC 和 ELISA 之间以及 ICC 和 PCR 之间存在实质性一致性。结果表明，骨髓 ICC 是 FeLV 感染的一种有效新型诊断测试。
