Department of Anatomy, College of Korean Medicine, Semyung University, Jecheon, Republic of Korea.
Translational Research Unit for Anatomy and Analgesia, Seoul, Republic of Korea.
Cells Tissues Organs. 2024;213(5):382-389. doi: 10.1159/000540976. Epub 2024 Aug 27.
Neurogenesis in the adult brain may play an important role in memory and cognition; however, knowledge of neurogenic markers in the human brain remains limited. We compared the single-nucleus transcriptome of the hippocampus with that of other cortical regions to identify hippocampus-specific neurogenic markers.
We analyzed 26,189 nuclei from four human brains collected within 16 h of death. Clustering and annotation were performed to examine differential expression, gene ontology, and intercellular communication. DCX expression was validated by ddPCR.
Immature markers such as DCX, CALB2, NES, SOX2, PAX6, DPYSL3, and TUBB3 were expressed in both hippocampus and prefrontal cortex, with higher levels in the prefrontal cortex. ddPCR confirmed higher expression of DCX in the prefrontal cortex. DCX was involved in both neurogenesis and neuroprotection pathways.
Neurogenic markers are not definitive indicators of adult neurogenesis as their roles are more complex than previously understood.
成人大脑中的神经发生可能在记忆和认知中发挥重要作用；然而，人类大脑中神经发生标记物的知识仍然有限。我们比较了海马体与其他皮质区域的单个核转录组，以鉴定海马体特异性神经发生标记物。
我们分析了 4 例在死亡后 16 小时内采集的人脑的 26189 个核。通过聚类和注释检查差异表达、基因本体和细胞间通讯。通过 ddPCR 验证 DCX 的表达。
未成熟标记物，如 DCX、CALB2、NES、SOX2、PAX6、DPYSL3 和 TUBB3，在海马体和前额叶皮质中均有表达，在前额叶皮质中表达水平更高。ddPCR 证实 DCX 在额皮质中的表达更高。DCX 参与神经发生和神经保护途径。
神经发生标记物并不是成人神经发生的明确指标，因为它们的作用比以前理解的更为复杂。
