• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

特林通过抑制恶性黑素瘤细胞中血管生成素表达发挥抗肿瘤活性。

Terrein Exhibits Anti-tumor Activity by Suppressing Angiogenin Expression in Malignant Melanoma Cells.

机构信息

Department of Oral and Maxillofacial Surgery, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama, Japan.

Department of Pharmacy, Faculty of Pharmacy, Gifu University of Medical Science, Gifu, Japan.

出版信息

Cancer Genomics Proteomics. 2024 Sep-Oct;21(5):464-473. doi: 10.21873/cgp.20464.

DOI:10.21873/cgp.20464
PMID:39191499
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11363929/
Abstract

BACKGROUND/AIM: Malignant melanoma is a tumor with a poor prognosis that can metastasize distally at an early stage. Terrein, a metabolite produced by Aspergillus terreus, suppresses the expression of angiogenin, an angiogenic factor. However, the pharmacological effects of natural terrein have not been elucidated, because only a small amount of terrein can be extracted from large fungal cultures. In this study, we investigated the antineoplastic effects of terrein on human malignant melanoma cells and its underlying mechanisms.

MATERIALS AND METHODS

Human malignant melanoma cell lines were cultured in the presence of terrein and analyzed. Angiogenin production was evaluated using ELISA. Ribosome biosynthesis was evaluated using silver staining of the nucleolar organizer region. Intracellular signaling pathways were analyzed using western blotting. Malignant melanoma cells were transplanted subcutaneously into the backs of nude mice. The tumors were removed at 5 weeks and analyzed histopathologically.

RESULTS

Terrein inhibited angiogenin expression, proliferation, migration, invasion, and ribosome biosynthesis in malignant melanoma cells. Terrein was shown to inhibit tumor growth and angiogenesis in animal models.

CONCLUSION

This study demonstrated that terrein has anti-tumor effects against malignant melanoma. Furthermore, chemically synthesized non-natural terrein can be mass-produced and serve as a novel potential anti-tumor drug candidate.

摘要

背景/目的：恶性黑色素瘤是一种预后不良的肿瘤，早期即可远处转移。土霉素是土曲霉产生的一种代谢产物，能抑制血管生成因子血管生成素的表达。然而，由于从大量真菌培养物中只能提取到少量土霉素，因此其药理学作用尚未阐明。本研究旨在探讨土霉素对人恶性黑素瘤细胞的抗肿瘤作用及其作用机制。

材料和方法

用土霉素培养人恶性黑素瘤细胞系并进行分析。用 ELISA 法评估血管生成素的产生。用核仁组成区银染法评估核糖体生物合成。用 Western blot 法分析细胞内信号通路。将恶性黑素瘤细胞皮下移植到裸鼠背部。5 周后切除肿瘤并进行组织病理学分析。

结果

土霉素抑制恶性黑素瘤细胞中的血管生成素表达、增殖、迁移、侵袭和核糖体生物合成。土霉素在动物模型中显示出抑制肿瘤生长和血管生成的作用。

结论

本研究表明土霉素对恶性黑色素瘤具有抗肿瘤作用。此外，化学合成的非天然土霉素可以大量生产，并可能成为一种新型的潜在抗肿瘤药物候选物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0374/11363929/006e1bdcb8a5/cgp-21-471-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0374/11363929/17285a539e4c/cgp-21-465-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0374/11363929/0145b321cd43/cgp-21-466-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0374/11363929/793c285602f4/cgp-21-469-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0374/11363929/6c292f9869bc/cgp-21-470-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0374/11363929/a311f9c1be55/cgp-21-471-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0374/11363929/006e1bdcb8a5/cgp-21-471-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0374/11363929/17285a539e4c/cgp-21-465-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0374/11363929/0145b321cd43/cgp-21-466-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0374/11363929/793c285602f4/cgp-21-469-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0374/11363929/6c292f9869bc/cgp-21-470-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0374/11363929/a311f9c1be55/cgp-21-471-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0374/11363929/006e1bdcb8a5/cgp-21-471-g0002.jpg

相似文献

1
Terrein Exhibits Anti-tumor Activity by Suppressing Angiogenin Expression in Malignant Melanoma Cells.特林通过抑制恶性黑素瘤细胞中血管生成素表达发挥抗肿瘤活性。
Cancer Genomics Proteomics. 2024 Sep-Oct;21(5):464-473. doi: 10.21873/cgp.20464.
2
Synthetic Terrein Inhibits Progression of Head and Neck Cancer by Suppressing Angiogenin Production.合成terrein通过抑制血管生成素的产生来抑制头颈癌的进展。
Anticancer Res. 2016 May;36(5):2161-8.
3
Basic fibroblast growth factor affects the expression of angiogenin and cell proliferation in A375 human melanoma cells.碱性成纤维细胞生长因子影响A375人黑素瘤细胞中血管生成素的表达和细胞增殖。
Tumori. 2011 Jan-Feb;97(1):95-103. doi: 10.1177/030089161109700117.
4
Inhibitory effects of terrein on lung cancer cell metastasis and angiogenesis.土曲霉毒素对肺癌细胞转移和血管生成的抑制作用。
Oncol Rep. 2021 Jun;45(6). doi: 10.3892/or.2021.8045. Epub 2021 Apr 13.
5
Terrein inhibits migration of human breast cancer cells via inhibition of the Rho and Rac signaling pathways. Terrein 通过抑制 Rho 和 Rac 信号通路抑制人乳腺癌细胞的迁移。
Oncol Rep. 2018 Mar;39(3):1378-1386. doi: 10.3892/or.2018.6189. Epub 2018 Jan 4.
6
The marine-derived fungal metabolite, terrein, inhibits cell proliferation and induces cell cycle arrest in human ovarian cancer cells.海洋来源的真菌代谢产物土曲霉毒素可抑制人卵巢癌细胞的增殖并诱导细胞周期停滞。
Int J Mol Med. 2014 Dec;34(6):1591-8. doi: 10.3892/ijmm.2014.1964. Epub 2014 Oct 13.
7
Neamine inhibits xenografic human tumor growth and angiogenesis in athymic mice.新霉素抑制无胸腺小鼠体内异种移植的人类肿瘤生长和血管生成。
Clin Cancer Res. 2005 Dec 15;11(24 Pt 1):8745-52. doi: 10.1158/1078-0432.CCR-05-1495.
8
A new terrein glucoside, a novel inhibitor of angiogenin secretion in tumor angiogenesis.一种新的土曲霉糖苷，一种肿瘤血管生成中血管生成素分泌的新型抑制剂。
J Antibiot (Tokyo). 2008 Jul;61(7):442-8. doi: 10.1038/ja.2008.60.
9
Neamine inhibits oral cancer progression by suppressing angiogenin-mediated angiogenesis and cancer cell proliferation.新霉素通过抑制血管生成素介导的血管生成和癌细胞增殖来抑制口腔癌进展。
Anticancer Res. 2014 May;34(5):2113-21.
10
ACTIBIND, a T2 RNase, competes with angiogenin and inhibits human melanoma growth, angiogenesis, and metastasis.ACTIBIND是一种T2核糖核酸酶，它与血管生成素竞争并抑制人类黑色素瘤的生长、血管生成和转移。
Cancer Res. 2007 Jun 1;67(11):5258-66. doi: 10.1158/0008-5472.CAN-07-0129.

本文引用的文献

1
NCCN Guidelines® Insights: Melanoma: Cutaneous, Version 2.2021.NCCN 指南®洞察：黑色素瘤：皮肤，第 2.2021 版。
J Natl Compr Canc Netw. 2021 Apr 1;19(4):364-376. doi: 10.6004/jnccn.2021.0018.
2
Adjuvant vemurafenib in resected, BRAF mutation-positive melanoma (BRIM8): a randomised, double-blind, placebo-controlled, multicentre, phase 3 trial.辅助维莫非尼治疗 BRAF 突变阳性黑色素瘤（BRIM8）：一项随机、双盲、安慰剂对照、多中心、III 期临床试验。
Lancet Oncol. 2018 Apr;19(4):510-520. doi: 10.1016/S1470-2045(18)30106-2. Epub 2018 Feb 21.
3
Plexin-B2 Mediates Physiologic and Pathologic Functions of Angiogenin.
丛状蛋白B2介导血管生成素的生理和病理功能。
Cell. 2017 Nov 2;171(4):849-864.e25. doi: 10.1016/j.cell.2017.10.005.
4
Adjuvant Nivolumab versus Ipilimumab in Resected Stage III or IV Melanoma.纳武利尤单抗辅助治疗与伊匹单抗用于切除的 III 期或 IV 期黑色素瘤。
N Engl J Med. 2017 Nov 9;377(19):1824-1835. doi: 10.1056/NEJMoa1709030. Epub 2017 Sep 10.
5
Semaphorin 4C Plexin-B2 signaling in peripheral sensory neurons is pronociceptive in a model of inflammatory pain.在炎性疼痛模型中，外周感觉神经元中的信号素4C-丛状蛋白-B2信号传导具有伤害感受作用。
Nat Commun. 2017 Aug 2;8(1):176. doi: 10.1038/s41467-017-00341-w.
6
Prolonged Survival in Stage III Melanoma with Ipilimumab Adjuvant Therapy.伊匹单抗辅助治疗Ⅲ期黑色素瘤的长期生存
N Engl J Med. 2016 Nov 10;375(19):1845-1855. doi: 10.1056/NEJMoa1611299. Epub 2016 Oct 7.
7
Synthetic Terrein Inhibits Progression of Head and Neck Cancer by Suppressing Angiogenin Production.合成terrein通过抑制血管生成素的产生来抑制头颈癌的进展。
Anticancer Res. 2016 May;36(5):2161-8.
8
From melanocytes to melanomas.从黑素细胞到黑色素瘤。
Nat Rev Cancer. 2016 Jun;16(6):345-58. doi: 10.1038/nrc.2016.37. Epub 2016 Apr 29.
9
Synthetic (+)-terrein suppresses interleukin-6/soluble interleukin-6 receptor induced-secretion of vascular endothelial growth factor in human gingival fibroblasts.合成（+）-土曲霉毒素抑制白细胞介素-6/可溶性白细胞介素-6受体诱导人牙龈成纤维细胞分泌血管内皮生长因子。
Bioorg Med Chem. 2014 Oct 1;22(19):5338-44. doi: 10.1016/j.bmc.2014.07.047. Epub 2014 Aug 7.
10
Cancer statistics, 2013.癌症统计数据，2013 年。
CA Cancer J Clin. 2013 Jan;63(1):11-30. doi: 10.3322/caac.21166. Epub 2013 Jan 17.