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红腿石鸡基因组的长读长从头组装。

Long-read de novo assembly of the red-legged partridge (Alectoris rufa) genome.

机构信息

Animal Breeding and Genomics, Wageningen University & Research, 6708PB, Wageningen, The Netherlands.

Departament de Ciència Animal, Universitat de Lleida, Lleida, Spain.

出版信息

Sci Data. 2024 Aug 22;11(1):908. doi: 10.1038/s41597-024-03659-5.

Abstract

The red-legged partridge (Alectoris rufa) is a popular game bird species that is in decline in several regions of southwestern Europe. The introduction of farm-reared individuals of a distinct genetic make-up in hunting reserves can result in genetic swamping of wild populations. Here we present a de novo genome assembly for the red-legged partridge based on long-read sequencing technology. The assembled genome size is 1.14 Gb, with scaffold N50 of 37.6 Mb and contig N50 of 29.5 Mb. Our genome is highly contiguous and contains 97.06% of complete avian core genes. Overall, the quality of this genome assembly is equivalent to those available for other close relatives such as the Japanese quail or the chicken. This genome assembly will contribute to the understanding of genetic dynamics of wild populations of red-legged partridges with releases of farm-reared reinforcements and to appropriate management decisions of such populations.

摘要

红腿石鸡(Alectoris rufa)是一种受欢迎的猎鸟物种,但在欧洲西南部的多个地区数量正在减少。在狩猎保护区引入具有明显遗传结构的人工饲养个体可能导致野生种群的遗传淹没。在这里,我们基于长读测序技术为红腿石鸡提供了一个从头组装的基因组。组装的基因组大小为 1.14Gb,支架 N50 为 37.6Mb,拼接 N50 为 29.5Mb。我们的基因组高度连续,包含 97.06%的完整鸟类核心基因。总的来说,这个基因组组装的质量与其他近亲(如日本鹌鹑或鸡)的基因组组装质量相当。这个基因组组装将有助于了解释放人工饲养的补充种群后红腿石鸡野生种群的遗传动态,并为这些种群的适当管理决策提供信息。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6f2/11349902/5c94e93c7fc2/41597_2024_3659_Fig1_HTML.jpg

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