Department of Physics and Energy, Chongqing University of Technology, Chongqing, 400054, China.
Institute of High Performance Computing, ASTAR S138632, Singapore, Singapore.
Mikrochim Acta. 2024 Aug 27;191(9):565. doi: 10.1007/s00604-024-06618-4.
A novel sandwich-type electrochemical immunosensor for the detection of the liver cancer marker alpha-fetoprotein (AFP) in human serum is proposed. The two-dimensional MXene material TiCT was first prepared using etching and ultrasonic stripping, and then TiCT was used to reduce chloroauric acid to form TiCT/AuNP composites which were modified on the surface of the glassy carbon electrodes to form probe-type sensors. The TiCT/AuNPs provide a large number of binding sites for the AFP capture antibody (Ab1) and increase the electrochemical reaction active site. The TiCT/copper metal-organic frameworks HKUST-1 composite was also prepared by solvothermal method and combined with toluidine blue (TB) and AFP detection antibody (Ab2) to form a labeled sandwich-type electrochemical immunosensor. The sensor achieved trace detection of AFP from 0.1 to 100 ng/mL with a detection limit of 0.073 pg/mL and possesses good selectivity, stability, and reproducibility. The sensor performs well in clinical samples and has good potential for clinical applications.
提出了一种用于检测人血清中肝癌标志物甲胎蛋白（AFP）的新型三明治型电化学免疫传感器。首先使用蚀刻和超声剥离制备二维 MXene 材料 TiCT，然后使用 TiCT 将氯金酸还原形成 TiCT/AuNP 复合材料，将其修饰在玻碳电极表面上形成探针型传感器。TiCT/AuNPs 为 AFP 捕获抗体（Ab1）提供了大量的结合位点，并增加了电化学反应活性位点。还通过溶剂热法制备了 TiCT/铜金属有机骨架 HKUST-1 复合材料，并与甲苯胺蓝（TB）和 AFP 检测抗体（Ab2）结合形成标记的三明治型电化学免疫传感器。该传感器实现了痕量 AFP 的检测，检测限为 0.073 pg/mL，检测范围为 0.1 至 100 ng/mL，具有良好的选择性、稳定性和重现性。该传感器在临床样本中表现良好，具有良好的临床应用潜力。
