Key Laboratory of Immune Response and Immunotherapy, Shanghai Institute of Immunity and Infection, Chinese Academy of Sciences, Shanghai, China.
Methods Mol Biol. 2025;2854:171-175. doi: 10.1007/978-1-0716-4108-8_17.
Phagocytosis is a central process by which macrophage cells internalize and eliminate microbes as well as apoptotic cells. The nascent phagosome undergoes a complex maturation process involving sequential fusion with endosomal compartments. The endosomal TLRs, including TLR3, -7, -8, and -9, play a critical role in innate immunity by sensing bacterial or viral nucleic acids and are preferentially transported to the phagosomal membrane of innate immune cells upon activation. Therefore, phagosome isolation is helpful for studies on pathogenic invasion and the functions of phagosome proteins, including endosomal TLRs.
吞噬作用是巨噬细胞内化和清除微生物和凋亡细胞的主要过程。初生吞噬体经历一个复杂的成熟过程，包括与内体隔室的连续融合。内体 TLR，包括 TLR3、-7、-8 和 -9，通过感应细菌或病毒核酸在天然免疫中发挥关键作用，并且在激活时优先被转运到天然免疫细胞的吞噬体膜上。因此，吞噬体分离有助于研究病原体入侵和吞噬体蛋白的功能，包括内体 TLR。
