[Aberrant Expression of Small Nucleolar RNA and Its Clinical Significance in Patients with Acute Leukemia].

OBJECTIVE

To investigate the expression level of small nucleolar RNA (snoRNA) in bone marrow of patients with acute leukemia (AL) and its significance in the clinical diagnosis, treatment and prognosis of AL patients.

METHODS

Bone marrow samples of 53 newly diagnosed AL patients and 29 healthy subjects in the Affiliated Hospital of Guangdong Medical University from March 2018 to December 2021 were collected. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to detect the relative expression level of in bone marrow mononuclear cells of the two groups. The median expression level of in AL patients was used as the boundary value to divide the patients into high and low expression groups, and the relationship between the expression level of and the clinical characteristics, clinical indicators and prognosis of AL patients was analyzed and discussed.

RESULTS

The relative expression level of in AL patients was significantly lower than that in healthy control group [0.3018 (0.0244-1.2792) 1.0882 (0.2797-1.9889)] ( < 0.01). The expression level of in AL patients without remission after initial treatment was significantly lower than that in healthy controls and the patients who received complete remission (CR) ( < 0.01), while there was no statistical difference in the expression level of between AML and ALL groups ( >0.05). The abnormal low expression of was closely related to fever, hemorrage, poor prognosis, efficacy, platelets (PLT), lactate dehydrogenase (LDH), albumin (ALB), and molecular biological abnormalities of AL patients ( < 0.05), but not significantly correlated with sex, age, AL subtype, pallor, fatigue, extramedullary infiltration, white blood cell count (WBC), hemoglobin (HGB), C-reactive protein (CRP), procalcitonin (PCT), fibrinogen (FIB) or chromosome karyotype ( >0.05). Meanwhile, overall survival (OS) and event-free survival (EFS) of AL patients in high-expression group were significantly higher than those in low-expression group ( < 0.05). Univariate Cox regression analysis showed that , molecular biological abnormalities, fever, PLT and LDH were the factors influencing OS and EFS in AL patients ( < 0.05). Multivariate Cox regression analysis indicated that fever, molecular biological abnormalities and LDH were independent factors associated with OS and EFS in AL patients ( < 0.05).

CONCLUSION

is significantly down-expressed in AL patients, which is a molecular marker of great clinical value for disease monitoring and prognosis evaluation in AL patients.