Liu Ai-Fei, Chen Yi-Jian
Department of Transfusion, The First Hospital Affiliated to Gannan Medical University, Ganzhou 341000, Jiangxi Province, China.
Department of Hematology, the First Hospital Affiliated to Gannan Medical University, Ganzhou 341000, Jiangxi Province, China,E-mail：
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2020 Dec;28(6):1819-1825. doi: 10.19746/j.cnki.issn.1009-2137.2020.06.005.
To determine the expression level of linc-223 and miR-125a in the patients with adult acute leukemia (AL) and explore the relationship between the expression level and the occurrence, development, prognosis of leukemia.
Bone marrow samples of 93 patients with AL treated in our hospital from January 2017 to September 2017 were enrolled, including 21 cases of acute lymphoblastic leukemia (ALL) and 72 cases of acute non-lymphocytic leukemia (ANLL). At the same time, bone marrow samples from 20 cases of non-malignant hematopathy patients in the same period were enrolled as control group. Real-time quantitative PCR (qRT-PCR) was used to test the expression level of linc-223 and miR-125a in bone marrow of 93 AL patients and the relationship between the level and the occurrence, development, prognosis of leukemia was analyzed.
There was no significant difference in the expression of linc-223 between AL patients (ANLL and ALL) and control group (P>0.05). Moreover, there was no significant correlation between linc-223 and PML-RARα gene or the remission rate of patients after treatment. The expression of miR-125a in ANLL patients was significantly lower than those in the control group (P<0.01), but there was no significant difference between the initial treatment, recurrence and remission group (P>0.05), and also for ALL and control group (P>0.05). In the newly treatment ANLL patients, the expression level of miR-125a showed negatively correlated with LDH level and the ratio of immature cells (r=-0.454, r=-0.400), but not with sex, degree of risk, peripheral blood leukocyte count, platelet count, hemoglobin content, WT1, CRP, etc. (P>0.05). There was a positive correlation between linc-223 and miR-125a in ANLL patients (r=0.296).
No abnormal expression of linc-223 was found in the bone marrow of AL patients, but miR-125a expression shows a low level and positively correlate with the expression level of linc-223 in ANLL, which is helpful for the diagnosis.
检测长链非编码RNA-223（linc-223）和微小RNA-125a（miR-125a）在成人急性白血病（AL）患者中的表达水平，探讨其表达水平与白血病发生、发展及预后的关系。
选取2017年1月至2017年9月在我院治疗的93例AL患者的骨髓样本，其中急性淋巴细胞白血病（ALL）21例，急性非淋巴细胞白血病（ANLL）72例。同时选取同期20例非恶性血液病患者的骨髓样本作为对照组。采用实时定量聚合酶链反应（qRT-PCR）检测93例AL患者骨髓中linc-223和miR-125a的表达水平，并分析其与白血病发生、发展及预后的关系。
AL患者（ANLL和ALL）与对照组linc-223表达差异无统计学意义（P>0.05）。且linc-223与PML-RARα基因及患者治疗后缓解率无明显相关性。ANLL患者miR-125a表达明显低于对照组（P<0.01），但初治、复发及缓解组间差异无统计学意义（P>0.05），ALL与对照组间差异亦无统计学意义（P>0.05）。初治ANLL患者中，miR-125a表达水平与乳酸脱氢酶（LDH）水平及幼稚细胞比例呈负相关（r=-0.454，r=-0.400），与性别、危险度、外周血白细胞计数、血小板计数、血红蛋白含量、WT1、CRP等无关（P>0.05）。ANLL患者中linc-223与miR-125a呈正相关（r=0.296）。
AL患者骨髓中未发现linc-223异常表达，但miR-125a表达水平较低，且在ANLL中与linc-223表达呈正相关，有助于诊断。
