Yang Yue, He Jian-Xin
Department of Medical Laboratory Technology, School of Medicine of Northwest University for Nationalities. Lanzhou 730000， Gansu Province, China.
Basic Medical College， Gansu University of Chinese Medicine. Lanzhou 730000， Gansu Province, China.
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2024 Aug;32(4):1224-1229. doi: 10.19746/j.cnki.issn.1009-2137.2024.04.039.
To explore whether Resveratrol (RSV) can inhibit the spontaneous senescence of human bone marrow-derived mesenchymal stem cells (MSC).
MSC were serially cultured to passage 13 and passage 15 to establish model groups exhibiting spontaneous senescence, respectively. MSC at passage 13 and passage 15 were treated with 5 nmol/L RSV for 48 h to establish the RSV-treated groups. SA-β-Gal staining was used to detect cell senescence. MTT assay was used to detect cell proliferation. RT-PCR method was used to detect senescenceassociated telomerase activity. Western blot was used to detect the senescence-associated protein level of the phosphorylated-mTOR.
SA-β-Gal staining showed that the senescent cells of MSC in RSV-treated group was significantly less than those in the model group (RSV group compared with model group at passage 13, < 0.05; RSV group compared with model group at passage 15, < 0.01). The cell proliferation ability of MSC in RSV-treated group was significantly higher than those in model group, at 72 h in passage 13, there was significant difference between RSV-treated group and model group ( < 0.05). RT-PCR results showed that the mRNA expression of MSC in RSV-treated group was higher than that in model group, which was significantly different between RSV-treated group and model group at passage 13 ( < 0.05). Western blot results showed that the phosphorylated (Ser2448)-mTOR level of MSC in RSV-treated group was lower than that in model group, which was significantly different between RSV-treated group and model group at passage 13 ( < 0.05).
RSV can inhibit the spontaneous senescence of human MSC by mediating mTOR activity.
探讨白藜芦醇（RSV）是否能抑制人骨髓间充质干细胞（MSC）的自然衰老。
将MSC连续培养至第13代和第15代，分别建立呈现自然衰老的模型组。将第13代和第15代的MSC用5 nmol/L RSV处理48小时，建立RSV处理组。采用SA-β-Gal染色检测细胞衰老。采用MTT法检测细胞增殖。采用RT-PCR法检测衰老相关端粒酶活性。采用蛋白质免疫印迹法检测磷酸化-mTOR的衰老相关蛋白水平。
SA-β-Gal染色显示，RSV处理组MSC的衰老细胞明显少于模型组（第13代时RSV组与模型组相比，<0.05；第15代时RSV组与模型组相比，<0.01）。RSV处理组MSC的细胞增殖能力明显高于模型组，在第13代培养72小时时，RSV处理组与模型组之间存在显著差异（<0.05）。RT-PCR结果显示，RSV处理组MSC的mRNA表达高于模型组，在第13代时RSV处理组与模型组之间存在显著差异（<0.05）。蛋白质免疫印迹结果显示，RSV处理组MSC的磷酸化（Ser2448）-mTOR水平低于模型组，在第13代时RSV处理组与模型组之间存在显著差异（<0.05）。
RSV可通过介导mTOR活性抑制人MSC的自然衰老。
