Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Mansoura University, Mansoura, Egypt.
Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Mansoura National University, Gamasa, Egypt.
Luminescence. 2024 Sep;39(9):e4877. doi: 10.1002/bio.4877.
Rifampicin is a frontline antibiotic in the management of tuberculosis. Since no spectrofluorimetric methods are reported for this drug, this approach was challenged to craft a sensitive, reliable, valid, fast, and green methodology. In recent years, fluorescence spectroscopy has received a lot of interest. Its benefits include ecological greenness and analytical performance. The pharmaceutical industries greatly like this approach because of its low energy and decreased solvent usage, which make it both economical and environmentally friendly. This methodology was based on utilizing the enhanced native fluorescence of the rifampicin at 341 nm after excitation at 241 nm in a beta-cyclodextrin micellar system. Modern developments in analytical chemistry have been applied to reduce risks to the workplace and environment by using distilled water as a dilution solvent for method application and optimization. The method was found excellent green with 97 eco-scale and 0.86 AGREE scores besides an 89.6 overall whiteness score. The range of linearity for rifampicin raw material was 0.2-1.5 μg·mL, and the average recoveries for raw material and spiked plasma were 100.15% and 99.64%, respectively. The suggested technique worked well for the commercial oral syrup of Rimactane® and did not conflict with any common additives.
利福平是治疗结核病的一线抗生素。由于目前尚未报道利福平的分光光度法，因此需要开发一种灵敏、可靠、有效、快速且绿色的方法。近年来，荧光光谱法受到了广泛关注。其优点包括生态绿色性和分析性能。制药行业非常喜欢这种方法，因为它的能耗低，溶剂用量少，既经济又环保。该方法基于在β-环糊精胶束体系中，在 241nm 激发下，利福平在 341nm 处产生增强的本征荧光。分析化学的现代发展已经应用于通过使用蒸馏水作为稀释溶剂来减少对工作场所和环境的风险，用于方法应用和优化。该方法的绿色程度很高，具有 97 生态标度和 0.86 的 AGREE 评分，以及 89.6 的整体白度评分。利福平原料药的线性范围为 0.2-1.5μg·mL，原料药和加标血浆的平均回收率分别为 100.15%和 99.64%。该方法适用于 Rimactane®的商业口服糖浆，且与任何常见添加剂均无干扰。
