Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Sohag University, Sohag, Egypt.
Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Assiut University, Assiut, Egypt.
Luminescence. 2024 Oct;39(10):e4917. doi: 10.1002/bio.4917.
This study introduces a remarkably simple, green, and highly sensitive inclusion complex based spectrofluorimetric method for analyzing two sodium glucose cotransporter-2 (SGLT2) inhibitors: empagliflozin (EGF) and dapagliflozin (DGF). The method utilizes beta-cyclodextrin (β-CD) complexation to enhance the native fluorescence of EGF and DGF in aqueous solutions, resulting in 11.0-fold and 9.0-fold intensity increases, respectively. Fluorescence measurements were conducted at 301 nm emission following 230 nm excitation for both drugs. The method demonstrates excellent linearity (0.9994 for EGF and 0.9993 for DGF) over concentration ranges of 5.0-250.0 ng/mL and 10.0-300.0 ng/mL, with low detection limits of 1.05 and 1.38 ng/mL for EGF and DGF, respectively. The method's versatility was validated through successful application in pharmaceutical formulations, content uniformity testing, and biological fluids. This eco-friendly approach primarily uses water as a solvent and requires minimal reagents. The method's environmental impact was comprehensively evaluated using the analytical eco-scale, green analytical procedure index (GAPI), and analytical greenness metric (AGREE).
本研究介绍了一种非常简单、绿色且高灵敏度的包含络合分光荧光法，用于分析两种钠-葡萄糖共转运蛋白 2（SGLT2）抑制剂：恩格列净（EGF）和达格列净（DGF）。该方法利用β-环糊精（β-CD）络合作用增强 EGF 和 DGF 在水溶液中的本征荧光，分别使强度增加了 11.0 倍和 9.0 倍。两种药物的荧光测量均在 230nm 激发后于 301nm 发射处进行。该方法在 5.0-250.0ng/mL 和 10.0-300.0ng/mL 的浓度范围内具有出色的线性（EGF 为 0.9994，DGF 为 0.9993），EGF 和 DGF 的检测限分别低至 1.05 和 1.38ng/mL。该方法通过成功应用于药物制剂、含量均匀度测试和生物体液中得到了验证。这种环保方法主要使用水作为溶剂，所需试剂极少。该方法的环境影响通过分析生态规模、绿色分析程序指数（GAPI）和分析绿色度指标（AGREE）进行了全面评估。
