Department of Research and Development, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj P.O. Box 31975/148, Iran.
Department of Human Bacterial Vaccine, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj P.O. Box 31975/148, Iran.
Arch Razi Inst. 2024 Feb 1;79(1):154-167. doi: 10.32592/ARI.2024.79.1.154. eCollection 2024 Feb.
Numerous species of venomous snakes of medical importance exist in Iran. (), one of the medically important snakes, also called the Persian horned viper, has a geographical spread that extends to the east, southwest, and central areas of Iran and is endemic across the wider region. As a result, this species is responsible for many snakebite occurrences. Venom from P. persicus found in the central province of Semnan contains phospholipase A2 and L-amino acid oxidase activities, and high toxic potency. The venom was fractionated by reverse-phase high-performance liquid chromatography (HPLC) and analyzed by Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), Western blotting and two-dimensional electrophoresis. Using liquid chromatography with tandem mass spectrometry (LC-MS/MS), a range of components were identified, consistent with the biochemical and toxicological properties of the venom. Proteins identified from 2D electrophoresis and shotgun methods included metallo- and serine proteases, phospholipases, oxidases, and Kunitz trypsin inhibitors, along with many other components at lower qualitative abundance. This study provides a more detailed understanding of the protein profile of Iranian venom, which can be effective in the production of an effective antidote against it. The analysis of the resulting data shows that there is a wide range of proteins in the venom of the Persian horned viper. This information can provide a better understanding of how venom is neutralized by polyclonal antivenom. Considering the wide presence of this snake and its related species in Iran and surrounding countries, knowing the venom protein profile of this family can be of great support to antivenom producers such as Razi Vaccine & Serum Research Institute in the preparation of regional antivenoms.
伊朗存在大量具有医学重要性的毒蛇物种。（），一种具有医学重要性的蛇，也被称为波斯角蝰，其地理分布范围延伸到伊朗的东部、西南部和中部地区，并在更广泛的地区流行。因此，这种蛇类导致了许多蛇咬伤事件。在伊朗中央省塞姆南发现的 P. persicus 毒液含有磷脂酶 A2 和 L-氨基酸氧化酶活性，具有很高的毒性。该毒液通过反相高效液相色谱 (HPLC) 进行分离，并通过十二烷基硫酸钠聚丙烯酰胺凝胶电泳 (SDS-PAGE)、Western blot 和二维电泳进行分析。使用液相色谱-串联质谱 (LC-MS/MS)，鉴定出一系列成分，与毒液的生化和毒理学特性一致。从 2D 电泳和鸟枪法中鉴定出的蛋白质包括金属蛋白酶和丝氨酸蛋白酶、磷脂酶、氧化酶和 Kunitz 胰蛋白酶抑制剂，以及许多其他定性丰度较低的成分。这项研究提供了对伊朗毒液蛋白质图谱的更详细了解，这对于生产有效的抗毒液可能是有效的。对所得数据的分析表明，波斯角蝰毒液中存在广泛的蛋白质。这些信息可以更好地了解多克隆抗蛇毒血清如何中和毒液。考虑到这种蛇及其在伊朗和周边国家的相关物种的广泛存在，了解这种蛇的毒液蛋白质图谱可以为拉齐疫苗和血清研究所等抗蛇毒血清生产商在制备区域抗蛇毒血清方面提供很大支持。
