Haglock-Adler Carrie J, Gruzdys Valentinas, Strathmann Frederick G
Agilent Technologies, Inc., Santa Clara, CA 95051, USA.
University of Utah School of Medicine, Salt Lake City, UT 84108, USA.
Clin Mass Spectrom. 2017 Nov 24;6:32-35. doi: 10.1016/j.clinms.2017.11.003. eCollection 2017 Dec.
This study aims to establish joint fluid reference levels for Chromium (Cr) and Cobalt (Co) in a reference population of available fluid types. Method performance was evaluated on an existing urine matrix calibration method using inductively coupled plasma-mass spectrometry. Method performance characteristics, including intra- and inter-assay imprecision, accuracy, linearity, AMR (analytical measurement range), sensitivity, and carryover were determined in accordance with clinical laboratory standards. Additionally, analytical and clinical recoveries were assessed to investigate comparability between available joint fluid types and existing calibrators prepared in urine to demonstrate acceptability of a matrix-substitution design. 124 de-identified joint fluid samples submitted for unrelated testing were used to establish the reference levels. Reference levels were determined, by using the 97.5th percentile, to be <20.4 µg/L for Cr and <29.9 µg/L for Co. In addition, the presented method overcomes the lack of an ample volume of joint fluid to use for matrix matched calibrators by employing a urine-based calibration curve. The data demonstrate acceptable matrix comparability.
本研究旨在为现有可用液体类型的参考人群建立铬（Cr）和钴（Co）的关节液参考水平。使用电感耦合等离子体质谱法在现有的尿液基质校准方法上评估方法性能。根据临床实验室标准确定方法性能特征，包括批内和批间不精密度、准确度、线性、分析测量范围（AMR）、灵敏度和残留。此外，评估分析回收率和临床回收率，以研究可用关节液类型与尿液中制备的现有校准物之间的可比性，以证明基质替代设计的可接受性。提交用于无关检测的124份身份不明的关节液样本用于建立参考水平。通过使用第97.5百分位数确定参考水平，铬为<20.4µg/L，钴为<29.9µg/L。此外，所提出的方法通过采用基于尿液的校准曲线克服了缺乏足够体积的关节液用于基质匹配校准物的问题。数据表明基质具有可接受的可比性。
