Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Jilin University, Changchun 130000, China.
State Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy Agricultural Sciences, Lanzhou 730000, China.
ACS Appl Mater Interfaces. 2024 Sep 11;16(36):47226-47241. doi: 10.1021/acsami.4c09309. Epub 2024 Aug 28.
The development of multifunctional wound adhesives is critical in clinical settings due to the scarcity of dressings with effective adhesive properties while protecting against infection by drug-resistant bacteria. Polysaccharide and gelatin-based hydrogels, known for their biocompatibility and bioactivity, assist in wound healing. This study introduces a multifunctional bioadhesive hydrogel developed through dynamic covalent bonding and light-triggered covalent bonding, comprising oxidized hyaluronic acid, methacrylated gelatin, and the bacteriocin recently discovered by our lab, named jileicin (JC). The adhesion strength of the hydrogel, measured at 180 kPa, was 4.35 times higher than that of the fibrin glue. Furthermore, the hydrogel demonstrated robust platelet adhesion, procoagulant activity, and outstanding hemostatic properties in a mouse liver injury model. Incorporating JC significantly enhanced the phagocytosis and bactericidal capabilities of the macrophages. This immunomodulatory function on host cells, coupled with its potent bacterial membrane-disrupting ability, makes JC an effective killer against methicillin-resistant . In wound repair experiments on diabetic mice with infected full-thickness skin defects, the hydrogel treatment group showed a notable reduction in bacterial load, accelerated M2-type macrophage polarization, diminished inflammation, and hastened wound healing. Owing to its outstanding biocompatibility, antibacterial activity, and controlled adhesion, this hydrogel presents a promising therapeutic option for treating infected skin wounds.
多功能伤口粘合剂的开发在临床环境中至关重要，因为具有有效粘合性能同时能防止耐药菌感染的敷料稀缺。基于多糖和明胶的水凝胶因其生物相容性和生物活性而有助于伤口愈合。本研究通过动态共价键和光触发共价键开发了一种多功能生物粘合剂水凝胶，该水凝胶由氧化透明质酸、甲基丙烯酰化明胶和我们实验室最近发现的细菌素命名为 jileicin (JC) 组成。水凝胶的粘合强度为 180 kPa，比纤维蛋白胶高 4.35 倍。此外，水凝胶在小鼠肝损伤模型中表现出强大的血小板黏附、促凝血活性和出色的止血性能。JC 的加入显著增强了巨噬细胞的吞噬和杀菌能力。这种对宿主细胞的免疫调节功能，加上其强大的细菌膜破坏能力，使 JC 成为对抗耐甲氧西林金黄色葡萄球菌的有效杀手。在糖尿病感染全层皮肤缺损小鼠的伤口修复实验中，水凝胶治疗组显示细菌负荷显著降低，M2 型巨噬细胞极化加速，炎症减轻，伤口愈合加快。由于其出色的生物相容性、抗菌活性和可控的粘合性，这种水凝胶为治疗感染性皮肤伤口提供了一种有前途的治疗选择。
