Shi Yimeng, Zhu Yan, Sun Jiaojiao, Yin Huancai, Yin Jian
School of Biomedical Engineering (Suzhou), Division of Life Sciences and Medicine, University of Science and Technology of China, Suzhou, Jiangsu 215163, PR China.
CAS Key Lab of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, Jiangsu 215163, PR China.
Analyst. 2024 Oct 7;149(20):5041-5051. doi: 10.1039/d4an00610k.
Nowadays, trace detection of thiram is in urgent demand due to its widespread application in agriculture and significant harmful effects on public health. In this work, a three-dimensional (3D) sea cucumber-like flexible porous surface-enhanced Raman scattering (SERS) substrate composed of a poly(vinylidene fluoride) (PVDF) membrane, ZnO nanorods, gold films, and Ag nanoparticles (Ag/Au/ZnO/P) has been established for the highly sensitive detection of thiram. The substrate takes advantage of the 3D morphology of the Ag/Au/ZnO system on a flexible porous PVDF membrane to produce abundant plasmonic hot spots. Meanwhile, the employment of an AgNPs/Au shell system combined the benefits of both gold and silver metals, thus guaranteeing stable and sensitive detection. With 4-mercaptobenzoic acid (4-MBA) as a probe molecule, the Ag/Au/ZnO/P substrate exhibited excellent linear detection in the range of 10-10 M, with a correlation coefficient () of 0.99 and an enhancement cofactor of 7.09 × 10. The substrate exhibited excellent uniformity with a related standard deviation (RSD) value of 3.82% and demonstrated high stability during a 15 d-storage test. In addition, the substrate could detect thiram in an aqueous solution at concentrations as low as 10 M with excellent selectivity. Meanwhile, thiram on the surface of apple peel could be easily detected by the Ag/Au/ZnO/P substrate with the "paste-and-peel" method in less than 10 s, and the detection limit could be as low as 0.48 ng cm. Overall, the remarkable performance of the Ag/Au/ZnO/P SERS substrate demonstrated its great potential for the environmental monitoring of thiram.
如今，由于福美双在农业中的广泛应用及其对公众健康的重大有害影响，对其进行痕量检测的需求十分迫切。在这项工作中，建立了一种由聚偏氟乙烯（PVDF）膜、ZnO纳米棒、金膜和银纳米颗粒（Ag/Au/ZnO/P）组成的三维（3D）海参状柔性多孔表面增强拉曼散射（SERS）基底，用于高灵敏度检测福美双。该基底利用柔性多孔PVDF膜上Ag/Au/ZnO体系的3D形态产生丰富的等离子体热点。同时，AgNPs/Au壳层体系的使用结合了金和银两种金属的优点，从而保证了稳定且灵敏的检测。以4-巯基苯甲酸（4-MBA）作为探针分子，Ag/Au/ZnO/P基底在10⁻¹⁰ M范围内表现出优异的线性检测，相关系数（r）为0.99，增强因子为7.09×10⁷。该基底具有优异的均匀性，相关标准偏差（RSD）值为3.82%，并在15天的储存测试中表现出高稳定性。此外，该基底能够以优异的选择性检测水溶液中低至10⁻⁹ M浓度的福美双。同时，采用“粘贴-剥离”方法，Ag/Au/ZnO/P基底能够在不到10秒的时间内轻松检测苹果皮表面的福美双，检测限低至0.48 ng/cm²。总体而言，Ag/Au/ZnO/P SERS基底的卓越性能证明了其在福美双环境监测方面的巨大潜力。
