Rothbard J B, Fernandez R, Wang L, Teng N N, Schoolnik G K
Proc Natl Acad Sci U S A. 1985 Feb;82(3):915-9. doi: 10.1073/pnas.82.3.915.
Antisera generated against each of seven synthetic peptides corresponding to constant and variable sequences of the pilin from gonococcal strain MS11 were assayed for their ability to crossreact with intact pili from both homologous and heterologous strains. The peptides elicited roughly equal antipeptide responses but varied substantially in their ability to elicit antisera that crossreacted with intact pili. Of the antisera to peptides corresponding to regions of conserved sequence, antisera directed against residues 69-84 were the most efficient in binding pili from all strains tested in both solid-phase assays and immunoblots. Anti-69-84 also efficiently precipitated a tryptic fragment of pilin known to bind human endocervical cells. Sera against the two peptides (121-134 and 135-151) previously shown to contain strain-specific epitopes crossreacted with MS11 pili equally well, but differed in their ability to bind pili from heterologous strains. Anti-121-134 was strain-specific whereas anti-135-151 bound all pilin tested. Each of the sera was examined for its ability to inhibit bacterial adhesion to a human endometrial carcinoma cell line. Sera generated against residues 41-50 and 69-84 successfully inhibited a heterologous gonococcal strain from binding. These peptides could be important components of an effective vaccine for the prevention of gonorrhea.
针对淋病奈瑟菌菌株MS11菌毛恒定和可变序列对应的七种合成肽分别产生的抗血清，检测了它们与同源和异源菌株完整菌毛交叉反应的能力。这些肽引发的抗肽反应大致相同，但在引发与完整菌毛交叉反应的抗血清的能力上有很大差异。在针对保守序列区域的肽的抗血清中，针对残基69 - 84的抗血清在固相分析和免疫印迹中与所有测试菌株的菌毛结合方面效率最高。抗69 - 84还能有效沉淀已知与人宫颈内膜细胞结合的菌毛胰蛋白酶片段。针对先前显示含有菌株特异性表位的两种肽（121 - 134和135 - 151）的血清与MS11菌毛的交叉反应同样良好，但在与异源菌株菌毛结合的能力上有所不同。抗121 - 134具有菌株特异性，而抗135 - 151能结合所有测试的菌毛蛋白。检测了每种血清抑制细菌黏附于人子宫内膜癌细胞系的能力。针对残基41 - 50和69 - 84产生的血清成功抑制了异源淋病奈瑟菌菌株的黏附。这些肽可能是预防淋病有效疫苗的重要组成部分。
