Cortese Yvonne J, Fayne Joanne, Colbert Declan M, Devine Declan M, Fogarty Andrew
PRISM Research Institute, Technological University of the Shannon: Midlands Midwest, N37 HD68 Athlone, Ireland.
Teleflex Medical EMEA, N37 EC90 Athlone, Ireland.
Biomimetics (Basel). 2024 Aug 14;9(8):491. doi: 10.3390/biomimetics9080491.
The aim of this study was to develop a novel biomimetic in vitro extraluminal migration model to observe the migration of bacteria along indwelling urinary catheters within the urethra and assess the efficacy of a prototype chlorhexidine diacetate (CHX) coating to prevent this migration. The in vitro urethra model utilised chromogenic agar. A catheter was inserted into each in vitro urethra. One side of the urethra was then inoculated with bacteria to replicate a contaminated urethral meatus. The models were then incubated for 30 days (d), with the migration distance recorded each day. Four indwelling catheter types were used to validate the in vitro urethra model and methodology. Using the biomimetic in vitro urethra model, and migrated the entire length of a control catheter within 24-48 h (h). In the presence of a prototype CHX coating, full migration of the channel was prevented for 30 d. The results of this study support the hypothesis that catheter-associated urinary tract infections (CAUTIs) could be prevented by targeting catheter-mediated extraluminal microbial migration from outside of the urinary tract into the bladder.
本研究的目的是开发一种新型的仿生体外管腔外迁移模型，以观察细菌沿尿道内留置导尿管的迁移情况，并评估一种原型双醋酸氯己定（CHX）涂层预防这种迁移的效果。体外尿道模型使用了显色琼脂。将一根导管插入每个体外尿道。然后在尿道的一侧接种细菌，以模拟受污染的尿道口。然后将模型孵育30天（d），每天记录迁移距离。使用四种留置导管类型来验证体外尿道模型和方法。使用仿生体外尿道模型，[此处原文缺失部分内容]在24至48小时（h）内迁移了一根对照导管的全长。在存在原型CHX涂层的情况下，通道的完全迁移在30天内得到了预防。本研究结果支持这样的假设，即通过针对导管介导的从尿道外进入膀胱的管腔外微生物迁移，可以预防导管相关尿路感染（CAUTIs）。
