Mubeen Hafiz Muhammad, Li Ying, Hu Chunmei
National Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China.
Biology (Basel). 2024 Jul 27;13(8):568. doi: 10.3390/biology13080568.
Pak choi exhibits a wide range of phenotypic and morphological variations, significantly impacting its carbohydrate composition. This study aimed to analyze these variations by employing UPLC-MS/MS technology on eight biological replicates of seven Pak choi cultivars. The untargeted metabolic analysis identified 513 metabolites, focusing on 16 key carbohydrates, including monosaccharides, disaccharides, and polysaccharides. Monosaccharides were the most prevalent, which were followed by di-, poly-, and oligosaccharides. Suzhouqing had the highest number of differentially accumulated metabolites (DAMs), while Xiangqingcai had the least. Notably, the cultivars Xiangqingcai, Suzhouqing, and Aijiaohuang showed significant metabolite differentiation. The study found 114 metabolites that differed significantly between Suzhouqing and Aijiaohuang, of which 69 were upregulated and 45 were downregulated. In Xiangqingcai and Aijiaohuang, 66 metabolites were upregulated and 49 were downregulated. Between Xiangqingcai and Suzhouqing, 80 metabolites were downregulated and 53 were upregulated. Key carbohydrate digestion and absorption pathways were identified alongside the most enriched flavonoid biosynthesis pathway in Xiangqingcai and Suzhouqing. The findings highlight the considerable carbohydrate variation among Pak choi cultivars, providing valuable insights for targeted carbohydrate extraction and improving nutritional and agricultural practices.
小白菜表现出广泛的表型和形态变异，对其碳水化合物组成有显著影响。本研究旨在通过对七个小白菜品种的八个生物学重复样本采用超高效液相色谱-串联质谱技术来分析这些变异。非靶向代谢分析鉴定出513种代谢物，重点关注16种关键碳水化合物，包括单糖、二糖和多糖。单糖最为普遍，其次是二糖、多糖和寡糖。苏州青的差异积累代谢物（DAM）数量最多，而香青菜最少。值得注意的是，香青菜、苏州青和矮脚黄品种表现出显著的代谢物分化。研究发现苏州青和矮脚黄之间有114种代谢物存在显著差异，其中69种上调，45种下调。在香青菜和矮脚黄之间，66种代谢物上调，49种下调。在香青菜和苏州青之间，80种代谢物下调，53种上调。同时还确定了关键的碳水化合物消化和吸收途径以及香青菜和苏州青中最丰富的黄酮类生物合成途径。这些发现突出了小白菜品种间碳水化合物的显著差异，为有针对性的碳水化合物提取以及改善营养和农业实践提供了有价值的见解。
