Machaliński Bogusław, Oszutowska-Mazurek Dorota, Mazurek Przemyslaw, Parafiniuk Mirosław, Szumilas Paweł, Zawiślak Alicja, Zaremba Małgorzata, Stecewicz Iwona, Zawodny Piotr, Wiszniewska Barbara
Department of General Pathology, Pomeranian Medical University in Szczecin, 70-111 Szczecin, Poland.
Department of Histology and Embryology, Pomeranian Medical University in Szczecin, 70-111 Szczecin, Poland.
Biology (Basel). 2024 Aug 20;13(8):636. doi: 10.3390/biology13080636.
Skin aging is a complex phenomenon influenced by multiple internal and external factors that can lead to significant changes in skin structure, particularly the degradation of key extracellular matrix (ECM) components such as collagen and elastic fibers in the dermis. In this study, we aimed to meticulously assess the morphological changes within these critical fibrous ECM elements in the dermis of the same volunteer at age 47 and 10 years later (2012 to 2022). Using advanced histological staining techniques, we examined the distribution and characteristics of ECM components, including type I collagen, type III collagen, and elastic fibers. Morphological analysis, facilitated by hematoxylin and eosin staining, allowed for an accurate assessment of fiber bundle thickness and a quantification of collagen and elastic fiber areas. In addition, we used the generalized Pareto distribution for histogram modeling to refine our statistical analyses. This research represents a pioneering effort to examine changes in ECM fiber material, specifically within the male dermis over a decade-long period. Our findings reveal substantial changes in the organization of type I collagen within the ECM, providing insight into the dynamic processes underlying skin aging.
皮肤老化是一种复杂的现象，受多种内部和外部因素影响，这些因素可导致皮肤结构发生显著变化，尤其是真皮中关键细胞外基质（ECM）成分的降解，如胶原蛋白和弹性纤维。在本研究中，我们旨在细致评估同一名志愿者在47岁时以及10年后（2012年至2022年）真皮中这些关键纤维状ECM成分的形态变化。我们使用先进的组织学染色技术，研究了ECM成分的分布和特征，包括I型胶原蛋白、III型胶原蛋白和弹性纤维。通过苏木精和伊红染色进行的形态学分析，能够准确评估纤维束厚度，并对胶原蛋白和弹性纤维区域进行量化。此外，我们使用广义帕累托分布进行直方图建模，以完善我们的统计分析。这项研究是首次对ECM纤维材料的变化进行研究，特别是在长达十年的时间里对男性真皮内的变化进行研究。我们的研究结果揭示了ECM中I型胶原蛋白组织的显著变化，为皮肤老化的动态过程提供了见解。
