Huang Li-Wen, Huang Tzu-Ching, Hu Yu-Chen, Hsieh Bau-Shan, Lin Jin-Seng, Hsu Han-Yin, Lee Chia-Chia, Chang Kee-Lung
Department of Medical Laboratory Science and Biotechnology, College of Health Sciences, Kaohsiung Medical University, Kaohsiung 807378, Taiwan.
Department of Biochemistry, School of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 807378, Taiwan.
Curr Issues Mol Biol. 2024 Aug 16;46(8):8969-8980. doi: 10.3390/cimb46080530.
Low-grade body inflammation is a major cause of osteoarthritis (OA), a common joint disease. Gut dysbiosis may lead to systemic inflammation which can be prevented by probiotic administration. The subsp. 557 (LDL557) has been demonstrated to have beneficial effects for anti-inflammation. This study investigated the effects of LDL557 on OA progress using monosodium iodoacetate (MIA)-induced OA of rats. Live or heat-killed (HK)-LDL557 of a low or high dose was administrated for two weeks before MIA-induced OA, and then continuously administrated for another six weeks. After taking supplements for eight weeks, OA progress was analyzed. Results showed that MIA induced knee joint swelling, chondrocyte damage, and cartilage degradation, and supplementation with a high dose of LDL557 reduced MIA-induced knee joint swelling, chondrocyte damage, and cartilage degradation. Additionally, MIA increased serum levels of the matrix-degrading enzyme MMP-13, while a high dose of HK-LDL557 decreased it for the controls. Simultaneously, bone turnover markers and inflammatory cytokines of serum were assayed, but no significant differences were found except for a TNF-α decrease from a low dose of live LDL557. These results demonstrated that supplementation with high doses of live LDL557 or HK-LDL557 can reduce the progression of MIA-induced OA in rats.
低度全身炎症是骨关节炎（OA）的主要病因，骨关节炎是一种常见的关节疾病。肠道菌群失调可能导致全身炎症，而益生菌给药可预防这种炎症。已证明亚种557（LDL557）具有抗炎有益作用。本研究使用碘乙酸钠（MIA）诱导的大鼠骨关节炎模型，研究了LDL557对骨关节炎进展的影响。在MIA诱导骨关节炎前两周给予低剂量或高剂量的活LDL557或热灭活（HK）-LDL557，然后持续给药六周。补充八周后，分析骨关节炎进展情况。结果显示，MIA诱导膝关节肿胀、软骨细胞损伤和软骨降解，高剂量LDL557补充剂可减轻MIA诱导的膝关节肿胀、软骨细胞损伤和软骨降解。此外，MIA增加了血清中基质降解酶MMP-13的水平，而高剂量的HK-LDL557可使其低于对照组。同时，检测血清中的骨转换标志物和炎性细胞因子，除低剂量活LDL557使TNF-α降低外，未发现显著差异。这些结果表明，高剂量的活LDL557或HK-LDL557补充剂可减轻MIA诱导的大鼠骨关节炎进展。
