Ge Shishuai, Chu Bo, Sun Xiaoting, Ma Jiajie, Yang Xianming, Wu Kongming
School of Resources and Environment, Henan Institute of Science and Technology, Xinxiang 453003, China.
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Insects. 2024 Jul 25;15(8):561. doi: 10.3390/insects15080561.
As a migratory invasive pest, (fall armyworm, FAW) has recently posed a serious threat to food security in newly invaded areas (especially in Africa and Asia). Understanding its migration (or dispersal) patterns in newly invaded areas is crucial for regional forecasting and management efforts. By screening an appropriate marking technique to conduct mark-release-recapture (MRR) experiments, the migration patterns of the FAW can be effectively studied. In this study, we added different concentrations of Calco Oil Red N-1700 (an oil-soluble marker) to a self-made artificial diet and assessed the rearing and marking efficacy. The results indicated that a concentration of 0.2% of Calco Oil Red N-1700 in the diet was optimal for marking adult FAWs. The biological indicators (e.g., developmental duration, reproductive parameters, and flight ability) of FAWs fed this diet were basically consistent with those of FAWs fed a normal diet, with a larval stage of 15.46 days, a pupal stage of 9.81 days, a pupal mass of 278.18 mg, an adult longevity of 15.41 days, and an egg deposition count of 1503.51. Meanwhile, the flight distance, duration, and velocity were 24.91 km, 7.16 h, and 3.40 km/h, respectively (12 h tethered-flight tests), without difference with the control. Females and males exhibited distinctive marking colors (red or pink) that persisted for at least 5 and 9 days, respectively. This study developed an economically effective internal marking method for the adult FAW, laying the foundation for conducting MRR experiments. This will help clarify the migration behavior and routes of the FAW, providing a scientific basis for formulating effective pest management strategies.
作为一种迁飞性入侵害虫，（草地贪夜蛾，FAW）最近对新入侵地区（特别是非洲和亚洲）的粮食安全构成了严重威胁。了解其在新入侵地区的迁飞（或扩散）模式对于区域预测和管理工作至关重要。通过筛选合适的标记技术进行标记释放再捕获（MRR）实验，可以有效地研究草地贪夜蛾的迁飞模式。在本研究中，我们将不同浓度的卡尔科油红N - 1700（一种油溶性标记物）添加到自制的人工饲料中，并评估饲养和标记效果。结果表明，饲料中卡尔科油红N - 1700的浓度为0.2%时最适合标记成年草地贪夜蛾。用这种饲料喂养的草地贪夜蛾的生物学指标（如发育历期、生殖参数和飞行能力）与用正常饲料喂养的草地贪夜蛾基本一致，幼虫期为15.46天，蛹期为9.81天，蛹重为278.18毫克，成虫寿命为15.41天，产卵量为1503.51粒。同时，飞行距离、持续时间和速度分别为24.91千米、7.16小时和3.40千米/小时（12小时系留飞行试验），与对照组无差异。雌性和雄性呈现出独特的标记颜色（红色或粉色），分别持续至少5天和9天。本研究开发了一种经济有效的成年草地贪夜蛾内部标记方法，为进行MRR实验奠定了基础。这将有助于阐明草地贪夜蛾的迁飞行为和路线，为制定有效的害虫管理策略提供科学依据。
