Turčinavičienė Jurga, Bernotienė Rasa, Petrašiūnas Andrius
Department of Zoology, Institute of Biosciences, Life Sciences Center of Vilnius University, Saulėtekio Av. 7, LT-10257 Vilnius, Lithuania.
Insects. 2024 Jul 30;15(8):581. doi: 10.3390/insects15080581.
Trypanosomatids from the Tabanidae family have not been studied in Lithuania in any detail. In this study, a nested PCR amplifying the DNA fragment coding the SSU rRNA was used to determine the spp. prevalence and diversity in the Tabanidae family collected in Lithuania in 2018-2019. In total, 101 Tabanidae individuals were investigated from six areas in Lithuania, and 14 different species were identified. The overall positivity of spp. DNA in tabanids was 50.5% (51/101). was the most abundant species and yielded the highest prevalence of trypanosomatids (84.62%, 22/26), while showed a high prevalence as well, reaching 77.8% (14/18). In flies of some species ( and ), was detected for the first time. Nine different haplotypes were detected as being distributed in different tabanid species. Analysis showed that most sequences obtained during our study were identical or extremely close to two major subclades: TthI and TthII. Our data analysis suggests the presence of different genotypes in the same tabanid species, meaning that different lineages of could be more related to the vertebrate host and not the fly species. This is the first study of trypanosomatid parasites in tabanids from Lithuania, and our results are valuable in providing data on the diversity of these parasites in different Tabanidae species.
立陶宛尚未对虻科的锥虫进行过详细研究。在本研究中，采用巢式聚合酶链反应扩增编码小亚基核糖体核糖核酸（SSU rRNA）的DNA片段，以确定2018 - 2019年在立陶宛采集的虻科昆虫中锥虫的物种流行率和多样性。总共从立陶宛的六个地区调查了101只虻科昆虫，鉴定出14个不同的物种。虻科昆虫中锥虫DNA的总体阳性率为50.5%（51/101）。某物种是最丰富的物种，其锥虫流行率最高（84.62%，22/26），而另一物种的流行率也很高，达到77.8%（14/18）。在某些物种（具体物种名未给出）的苍蝇中，首次检测到了某锥虫。检测到九个不同的单倍型分布在不同的虻科物种中。分析表明，我们研究中获得的大多数序列与两个主要的锥虫亚分支：TthI和TthII相同或极其接近。我们的数据分析表明，在同一虻科物种中存在不同的锥虫基因型，这意味着锥虫的不同谱系可能与脊椎动物宿主的关系更大，而不是与苍蝇物种的关系。这是立陶宛对虻科昆虫中锥虫寄生虫的首次研究，我们的结果对于提供这些寄生虫在不同虻科物种中的多样性数据具有重要价值。
