Khadbaatar Solongo, Bao Haiying, Gao Xusheng, Huo Huimin
Key Laboratory of Edible Fungi Resources and Utilization, Ministry of Agriculture and Rural Affairs, Jilin Agricultural University, Changchun 130118, China.
Botanical Garden and Research Institute, Mongolian Academy of Science, Ulaanbaatar 13330, Mongolia.
J Fungi (Basel). 2024 Jul 27;10(8):524. doi: 10.3390/jof10080524.
In traditional Chinese medicine, Ganoderma is a kind of edible and medicinal mushroom, which is widely used because of its significant pharmacological activity. There are many species within the genus, each with different material bases and applications. However, detailed studies on these species are still lacking. In this study, we investigated the metabolites of (B), (C), (S) from Changbai Mountain, and (M) from Mongolia using metabolomics. The PCA results indicated minimal differences between M and S, whereas B and S exhibited significant variations. A total of 708 differential metabolites were identified in this study, with steroids, triterpenoids, phenols, and quinones being the major metabolites. Specifically, triterpenoids and steroids were higher in C. Meanwhile, phenolic compounds were more abundant in B. Additionally, quinones were more abundant in M and S. We validated some of the main compounds, and the results showed that paracetamol was most abundant in B, making paracetamol a potential marker for identifying B. Additionally, vitamin K3 was found to be more abundant in M and S, which can serve as a marker for their identification. This study provides new insights and a theoretical basis for the development and utilization of the genus .
在传统中医中，灵芝是一种药食两用的真菌，因其显著的药理活性而被广泛应用。该属内有许多物种，每个物种都有不同的物质基础和应用。然而，对这些物种的详细研究仍然不足。在本研究中，我们使用代谢组学方法研究了来自长白山的（B）、（C）、（S）以及来自蒙古的（M）的代谢产物。主成分分析（PCA）结果表明，M和S之间差异最小，而B和S表现出显著差异。本研究共鉴定出708种差异代谢产物，其中类固醇、三萜类、酚类和醌类是主要代谢产物。具体而言，三萜类和类固醇在C中含量较高。同时，酚类化合物在B中更为丰富。此外，醌类在M和S中含量更高。我们对一些主要化合物进行了验证，结果表明对乙酰氨基酚在B中含量最高，使其成为鉴定B的潜在标志物。此外，发现维生素K3在M和S中含量更高，可作为它们的鉴定标志物。本研究为该属的开发利用提供了新的见解和理论依据。
