Lemos Michele Scardine Corrêa de, Higa Junior Minoru German, Paniago Anamaria Mello Miranda, Melhem Marcia de Souza Carvalho, Takahashi Juliana Possato Fernandes, Fava Wellington Santos, Venancio Fabio Antonio, Martins Nayara Moreno, Chang Marilene Rodrigues
Graduate Program in Infectious and Parasitic Diseases, Faculty of Medicine, Federal University of Mato Grosso do Sul, Campo Grande 79070-900, MS, Brazil.
Hospital Infection Control Commission, Maria Aparecida Pedrossian University Hospital, Federal University of Mato Grosso do Sul, Campo Grande 79070-900, MS, Brazil.
J Fungi (Basel). 2024 Aug 1;10(8):538. doi: 10.3390/jof10080538.
Airborne spp. are critical pathogens that cause nosocomial infections in hospitals. Despite their importance, little is known about the distribution of species in the indoor air of hospitals in Brazil. We investigated spp. in the indoor air of critical areas in a tertiary hospital in Brazil. Air samples (n = 238) were collected from the intensive care unit (ICU), medical clinic unit (MCU), and urgency and emergency unit (UEU) using an air sampler (100 L/min). Of the 324 isolates, 322 were identified using phenotypic methods, and 37 were identified using DNA sequencing. spp. was grouped into five sections: (29.3%), (27.8%), (27.5%), (11.7%), and (3.1%). The predominant species identified via sequencing were (n = 9), (n = 7), and (n = 6). The number of spp. and their sections varied according to the collection day. was isolated more frequently during winter and in the ICU. This study is the first to demonstrate the diversity of airborne (saprophytic, allergenic, toxigenic, and potentially pathogenic) strains in a hospital located in the Midwest region of Brazil. It contributes to the knowledge of the diversity of cryptic species in the hospital environment.
空气传播的菌种是导致医院内医院感染的关键病原体。尽管它们很重要，但对于巴西医院室内空气中菌种的分布情况却知之甚少。我们对巴西一家三级医院关键区域的室内空气中的菌种进行了调查。使用空气采样器（100升/分钟）从重症监护病房（ICU）、内科门诊单元（MCU）和急诊单元（UEU）采集了空气样本（n = 238）。在324株分离菌株中，322株通过表型方法鉴定，37株通过DNA测序鉴定。菌种被分为五个部分：第一部分（29.3%）、第二部分（27.8%）、第三部分（27.5%）、第四部分（11.7%）和第五部分（3.1%）。通过测序鉴定出的主要菌种为第一部分（n = 9）、第二部分（n = 7）和第三部分（n = 6）。菌种及其部分的数量根据采集日期而有所不同。在冬季和ICU中分离出的该菌种更为频繁。本研究首次证明了位于巴西中西部地区一家医院中空气传播的该菌种（腐生、致敏、产毒和潜在致病）菌株的多样性。它有助于了解医院环境中隐性菌种的多样性。
