Laboratoire de Parasitologie-Mycologie, Hôpital Lariboisière Saint-Louis Fernand Widal, Assistance Publique-Hôpitaux de Paris (AP-HP), France.
Institut Pasteur, CNRS, Unité de Mycologie Moléculaire, Centre National de Référence Mycologie et Antifongiques, UMR2000, France.
Med Mycol. 2021 Oct 4;59(10):985-992. doi: 10.1093/mmy/myab027.
Black aspergilli of the section Nigri are rarely differentiated at the species level when originating from human specimens. We wondered whether some cryptic species could be more frequently observed in some clinical entities. We analyzed the 198 black isolates consecutively collected from the external ear canal (EEC; n = 66), respiratory specimens (n = 99), and environment (n = 33). DNA was extracted and species identification was performed upon the partial calmodulin gene. We identified by decreasing frequency: Aspergillus welwitschiae (35.3%), Aspergillus tubingensis (34.3%), Aspergillus niger (17.2%), Aspergillus luchuensis (4%), Aspergillus aff. welwitschiae (3%), Aspergillus neoniger (2%), Aspergillus piperis (1.5%), Aspergillus japonicus (1.0%), Aspergillus vadensis (0.5%), and two Aspergillus tubingensis clade (1%). The distribution of the three main cryptic species was different between EEC and respiratory samples (P < 0.001) but not different between respiratory and environment samples (P = 0.264). Aspergillus welwitschiae was more often associated with EEC (54.5%), whereas A. tubingensis and A. niger were predominant in respiratory samples (39.4 and 26.3%, respectively). Among the 99 respiratory isolates, only 10 were deemed responsible for probable invasive aspergillosis, of which six were mixed with other pathogenic moulds. This study shows the interest to pursue the identification of clinical isolates in the Aspergillus section Nigri to unravel some specific associations with clinical entities. The association of A. welwitschiae with otomycosis suggests a better fitness to infect/colonize the ear canal. Also, members of the Aspergillus section Nigri alone are rarely responsible for invasive aspergillosis.
We analyzed 198 black aspergilli isolates collected from different samples type to determine their species identification. We observe a different distribution of species between ear canal and respiratory samples (P < 0.001), suggesting a better fitness of A. welwitschiae to infect the ear canal.
当黑曲霉种的节 Nigri 来源于人体标本时，很少在种的水平上进行区分。我们想知道某些隐种是否会在某些临床实体中更频繁地被观察到。我们分析了从外耳（EEC；n=66）、呼吸道标本（n=99）和环境（n=33）连续收集的 198 个黑曲霉分离株。提取 DNA 并通过部分钙调蛋白基因进行种鉴定。我们按频率降低的顺序鉴定：aspergillus welwitschiae（35.3%）、aspergillus tubingensis（34.3%）、aspergillus niger（17.2%）、aspergillus luchuensis（4%）、aspergillus aff. welwitschiae（3%）、aspergillus neoniger（2%）、aspergillus piperis（1.5%）、aspergillus japonicus（1.0%）、aspergillus vadensis（0.5%）和 2 个 aspergillus tubingensis 分支（1%）。EEC 和呼吸道样本之间三种主要隐种的分布不同（P<0.001），但呼吸道和环境样本之间的分布无差异（P=0.264）。aspergillus welwitschiae 更常与 EEC 相关（54.5%），而 A. tubingensis 和 A. niger 则在呼吸道样本中占主导地位（分别为 39.4%和 26.3%）。在 99 个呼吸道分离株中，只有 10 个被认为可能导致侵袭性曲霉病，其中 6 个与其他致病性霉菌混合。这项研究表明，有必要继续鉴定黑曲霉节中的临床分离株，以揭示与临床实体的某些特定关联。aspergillus welwitschiae 与耳霉菌病的关联表明其更适合感染/定植耳道。此外，黑曲霉节的成员单独很少导致侵袭性曲霉病。
我们分析了从不同样本类型收集的 198 个黑曲霉分离株，以确定它们的种鉴定。我们观察到种在耳道和呼吸道样本之间的分布不同（P<0.001），这表明 A. welwitschiae 更适合感染耳道。
