Li Shuxian, Hu Xiaojun, Song Qijian
United States Department of Agriculture, Agricultural Research Service (USDA, ARS), Crop Genetics Research Unit, 141 Experiment Station Rd., Stoneville, MS 38776, USA.
USDA, Animal and Plant Health Inspection Service (APHIS), Plant Protection and Quarantine (PPQ), Plant Germplasm Quarantine Program (PGQP), Beltsville, MD 20708, USA.
J Fungi (Basel). 2024 Aug 13;10(8):570. doi: 10.3390/jof10080570.
(syn. ) is an important seed-borne fungal pathogen and the primary cause of Phomopsis seed decay (PSD) in soybean. PSD is one of the most devastating seed diseases, reducing soybean seed quality and yield worldwide. As part of a genome sequencing project on the fungal complex, draft genomes of eight isolates were sequenced and assembled. Sequences of mitochondrial genomes were extracted and analyzed. The circular mitochondrial genomes ranged from 52,534 bp to 58,280 bp long, with a mean GC content of 34%. A total of 14 core protein-coding genes, 23 tRNA, and 2 rRNA genes were identified. Introns were detected in the genes of , , , , , , , , and . Three isolates (PL7, PL10, and PL185E) had more introns than other isolates. Approximately 6.4% of the mitochondrial genomes consist of repetitive elements. Moreover, 48 single-nucleotide polymorphisms (SNPs) and were identified. The mitochondrial genome sequences of will be useful to further study the molecular basis of seed-borne pathogens causing seed diseases, investigate genetic variation among isolates, and develop improved control strategies for Phomopsis seed decay of soybean.
（同义词）是一种重要的种传真菌病原体，也是大豆拟茎点霉种子腐烂病（PSD）的主要病因。PSD是最具破坏性的种子病害之一，在全球范围内降低了大豆种子的质量和产量。作为该真菌复合体基因组测序项目的一部分，对8个分离株的基因组草图进行了测序和组装。提取并分析了线粒体基因组序列。环状线粒体基因组长度在52,534 bp至58,280 bp之间，平均GC含量为34%。共鉴定出14个核心蛋白质编码基因、23个tRNA和2个rRNA基因。在内含子、、、、、、、和的基因中检测到内含子。三个分离株（PL7、PL10和PL185E）的内含子比其他分离株多。线粒体基因组约6.4%由重复元件组成。此外，还鉴定出48个单核苷酸多态性（SNP）。的线粒体基因组序列将有助于进一步研究引起种子病害的种传病原体的分子基础，调查分离株之间的遗传变异，并制定改进的大豆拟茎点霉种子腐烂病防治策略。
