Centre des Sciences du Goût et de l'Alimentation, CNRS, INRAe, Institut Agro, Université de Bourgogne, F-21000 Dijon, France.
Institute for Neuro- and Behavioral Biology, University of Münster, 48149 Münster, Germany.
Cells. 2024 Aug 13;13(16):1340. doi: 10.3390/cells13161340.
The proper functioning of neural circuits that integrate sensory signals is essential for individual adaptation to an ever-changing environment. Many molecules can modulate neuronal activity, including neurotransmitters, receptors, and even amino acids. Here, we ask whether amino acid transporters expressed by neurons can influence neuronal activity. We found that (), which encodes a light chain of a heterodimeric amino acid transporter, is expressed in different cell types of the adult brain: in mushroom body neurons (MBs) and in glial cells. Using live calcium imaging, we found that MND expressed in α/β MB neurons is essential for sensitivity to the L-amino acids: Leu, Ile, Asp, Glu, Lys, Thr, and Arg. We found that the Target Of Rapamycin (TOR) pathway but not the Glutamate Dehydrogenase (GDH) pathway is involved in the Leucine-dependent response of α/β MB neurons. This study strongly supports the key role of MND in regulating MB activity in response to amino acids.
神经元中整合感觉信号的神经回路的正常运作对于个体适应不断变化的环境至关重要。许多分子可以调节神经元活动，包括神经递质、受体，甚至氨基酸。在这里，我们想知道神经元表达的氨基酸转运体是否可以影响神经元活性。我们发现，编码异二聚体氨基酸转运体轻链的（）在成年大脑的不同细胞类型中表达：在蘑菇体神经元（MBs）和神经胶质细胞中。使用活钙成像，我们发现表达在α/β MB 神经元中的 MND 对于亮氨酸、异亮氨酸、天冬氨酸、谷氨酸、赖氨酸、苏氨酸和精氨酸等 L-氨基酸的敏感性是必需的。我们发现雷帕霉素靶蛋白（TOR）通路而不是谷氨酸脱氢酶（GDH）通路参与了 α/β MB 神经元中亮氨酸依赖的反应。这项研究强烈支持 MND 在调节 MB 对氨基酸的活性反应中的关键作用。
