Guangdong Technology Research Center for Marine Algal Bioengineering, Guangdong Key Laboratory of Plant Epigenetics, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518060, China.
Ecological Fertilizer Research Institute, Shenzhen Batian Ecological Engineering Co., Ltd., Shenzhen 518057, China.
Mar Drugs. 2024 Aug 16;22(8):371. doi: 10.3390/md22080371.
is a well-known long-chain polyunsaturated fatty acids (PUFAs) producer, especially docosahexaenoic acid (DHA). In order to reduce the cost or improve the productivity of DHA, many researchers are focusing on exploring the high-yield strain, reducing production costs, changing culture conditions, and other measures. In this study, DHA production was improved by a two-stage fermentation. In the first stage, efficient and cheap soybean powder was used instead of conventional peptone, and the optimization of fermentation conditions (optimal fermentation conditions: temperature 28.7 °C, salinity 10.7‱, nitrogen source concentration 1.01 g/L, and two-nitrogen ratio of yeast extract to soybean powder 2:1) based on response surface methodology resulted in a 1.68-fold increase in biomass concentration. In the second stage, the addition of 2.5 mM sesamol increased the production of fatty acid and DHA by 93.49% and 98.22%, respectively, as compared to the optimal culture condition with unadded sesamol. Transcriptome analyses revealed that the addition of sesamol resulted in the upregulation of some genes related to fatty acid synthesis and antioxidant enzymes in . This research provides a low-cost and effective culture method for the commercial production of DHA by sp.
是一种众所周知的长链多不饱和脂肪酸（PUFAs）生产菌，尤其擅长生产二十二碳六烯酸（DHA）。为了降低 DHA 的成本或提高其生产力，许多研究人员专注于探索高产菌株、降低生产成本、改变培养条件等措施。在本研究中，采用两段式发酵来提高 DHA 的产量。在第一阶段，使用高效且廉价的大豆粉替代常规的蛋白胨，并基于响应面法优化发酵条件（最佳发酵条件：温度 28.7°C，盐度 10.7‱，氮源浓度 1.01 g/L，酵母提取物与大豆粉的双氮比为 2:1），这使得生物量浓度提高了 1.68 倍。在第二阶段，添加 2.5 mM 芝麻酚使脂肪酸和 DHA 的产量分别增加了 93.49%和 98.22%，与未添加芝麻酚的最佳培养条件相比。转录组分析表明，添加芝麻酚导致 sp. 中一些与脂肪酸合成和抗氧化酶相关的基因上调。本研究为 sp. 商业化生产 DHA 提供了一种低成本、有效的培养方法。
