DBT-ICT Centre for Energy Biosciences, Institute of Chemical Technology, Mumbai, India.
DBT-ICT Centre for Energy Biosciences, Institute of Chemical Technology, Mumbai, India.
Bioresour Technol. 2022 Feb;346:126544. doi: 10.1016/j.biortech.2021.126544. Epub 2021 Dec 11.
Marine protist Aurantiochytrium limacinum produces docosahexaenoic acid (DHA) as main polyunsaturated fatty acid (PUFA) and lacks any monounsaturated fatty acids (MUFA), while eicosapentaenoic acid (EPA) and MUFA's are produced by Phaeodactylum tricornutum. The marine diatom P. tricornutum was co-cultured with A.limacinum to match the EPA:DHA ratio of fish oil. Modulation in initial cell density ratio overcame the dominance of A.limacinum during co-cultivation and led to regulated proliferation of both species. Media engineering with nitrate and glycerol concentration yielded 2:1 (56.44: 30.11) mg g and 1:1 (47.43: 49.61) mg g EPA: DHA ratio. The oil and biomass obtained from co-cultivation comprised of MUFA's such as palmitoleic acid (2.65 mg g) and oleic acid (1.25 mg g) along with pigments like fucoxanthin (367.18 µg g), β-carotene (8.98 µg g) and astaxanthin (0.77 µg g). Thus, co-cultivation of P. tricornutum with A. limacinum represented a unique strategy towards achieving desired fatty acid composition.
海洋原生生物节旋藻产生二十二碳六烯酸（DHA）作为主要的多不饱和脂肪酸（PUFA），而不含有任何单不饱和脂肪酸（MUFA），而磷虾油则含有二十碳五烯酸（EPA）和 MUFA。海洋硅藻三角褐指藻与节旋藻共培养以匹配鱼油的 EPA:DHA 比例。通过初始细胞密度比值的调节克服了共培养过程中节旋藻的优势，并导致两种生物的有规律增殖。通过硝酸盐和甘油浓度的介质工程，可以得到 2:1（56.44:30.11）mg g 和 1:1（47.43:49.61）mg g 的 EPA:DHA 比值。共培养获得的油和生物量包含 MUFA，如棕榈油酸（2.65mg g）和油酸（1.25mg g）以及类胡萝卜素，如岩藻黄质（367.18µg g）、β-胡萝卜素（8.98µg g）和虾青素（0.77µg g）。因此，三角褐指藻与节旋藻的共培养代表了一种实现所需脂肪酸组成的独特策略。
