Instituto Politécnico Nacional, Escuela Nacional de Ciencias Biológicas, Laboratorio de Hidrobiología Experimental, Carpio y Plan de Ayala s/n, Ciudad de México 11340, Mexico.
Toxins (Basel). 2024 Aug 15;16(8):360. doi: 10.3390/toxins16080360.
Anthropic eutrophication leads to water quality degradation because it may cause the development of harmful cyanobacterial blooms, affecting aquatic biota and threatening human health. Because in the natural environment zooplankters are exposed continuously or intermittently to cyanotoxins in the water or through cyanobacterial consumption, this study aimed to assess the effects of the toxigenic VU-5 by different ways of exposure in . The acute toxicity produced by the cells, the aqueous crude extract of cells (ACE), and the cell-free culture medium (CFM) were determined. The effect on the survival and reproduction of under continuous and intermittent exposure was determined during 26 d. The LC was 407,000 cells mL; exposure to the ACE and CFM produced mortality lower than 20%. survivorship and reproduction were significantly reduced. Continuous exposure to cells caused 100% mortality on the fourth day. Exposure during 4 and 24 h in 48 h cycles produced adult mortality, and reproduction decreased as the exposure time and the concentrations increased. The higher toxicity of cells than the ACE could mean that the toxin's absorption is higher in the digestive tract. The temporary exposure to cells produced irreversible damage despite the recovery periods with microalgae as food. The form and the continuity in exposure to produced adverse effects, warning about threats to the zooplankton during HCBs.
人为富营养化会导致水质恶化,因为它可能导致有害蓝藻水华的发展,影响水生生物群和威胁人类健康。因为在自然环境中,浮游动物会持续或间歇性地暴露于水中的蓝藻毒素或通过食用蓝藻,所以本研究旨在评估不同暴露方式下产毒 VU-5 对 的影响。通过细胞、细胞水提粗提物(ACE)和无细胞培养液(CFM)来确定其急性毒性。在 26 天内,通过连续和间歇暴露来确定对 生存和繁殖的影响。LC 为 407,000 个细胞 mL;暴露于 ACE 和 CFM 会导致死亡率低于 20%。 存活率和繁殖力显著降低。连续暴露于细胞会在第四天导致 100%的死亡率。在 48 小时的周期中,进行 4 和 24 小时的暴露会导致成虫死亡,并且随着暴露时间和 浓度的增加,繁殖力下降。细胞的毒性比 ACE 高,这可能意味着毒素在消化道中的吸收更高。尽管有以微藻为食的恢复期,但短暂暴露于细胞会产生不可逆转的损伤。暴露形式和连续性会对浮游动物产生不利影响,这就警告了在 HCB 期间浮游动物所面临的威胁。