Kapoor Sheena, Sen Sushmita, Tsang Josephine, Yap Qi-Jing, Park Stanley, Cromarty Jerry, Swartzfager Deanna, Choy Kevin, Lim Sungwon, Koo Jamin, Holcomb Ilona
ImpriMed, Inc., 1130 Independence Avenue, Mountain View, CA 94043, USA.
BluePearl Pet Hospital, Kirkland, WA 98034, USA.
Vet Sci. 2024 Jul 24;11(8):331. doi: 10.3390/vetsci11080331.
Feline lymphoma, a prevalent cancer in cats, exhibits varied prognoses influenced by anatomical site and cellular characteristics. In this study, we investigated the utility of flow cytometry and clonality analysis via PCR for antigen receptor rearrangement (PARR) with respect to characterizing the disease and predicting prognosis. For this purpose, we received fine needle aspirates and/or blood from 438 feline patients, which were subjected to flow cytometry analysis and PARR. We used a subset of the results from patients with confirmed B- or T-cell lymphomas for comparison to cytological or histological evaluation (n = 53). Using them as a training set, we identified the optimal set of flow cytometry parameters, namely forward scatter thresholds, for cell size categorization by correlating with cytology-defined sizes. Concordance with cytological sizing among this training set was 82%. Furthermore, 90% concordance was observed when the proposed cell sizing was tested on an independent test set (n = 24), underscoring the reliability of the proposed approach. Additionally, lymphoma subtypes defined by flow cytometry and PARR demonstrated significant survival differences, validating the prognostic utility of these methods. The proposed methodology achieves high concordance with cytological evaluations and provides an additional tool for the characterization and management of feline lymphoproliferative diseases.
猫淋巴瘤是猫科动物中一种常见的癌症，其预后因解剖部位和细胞特征而异。在本研究中，我们调查了流式细胞术和通过聚合酶链反应（PCR）进行抗原受体重排克隆性分析（PARR）在疾病特征描述和预后预测方面的实用性。为此，我们收集了438只猫科动物患者的细针穿刺抽吸物和/或血液，对其进行流式细胞术分析和PARR。我们使用确诊为B细胞或T细胞淋巴瘤患者的一部分结果与细胞学或组织学评估结果进行比较（n = 53）。以这些结果作为训练集，我们通过与细胞学定义的大小相关联，确定了用于细胞大小分类的流式细胞术最佳参数集，即前向散射阈值。该训练集与细胞学大小的一致性为82%。此外，当在独立测试集（n = 24）上测试所提出的细胞大小分类方法时，观察到一致性为90%，这突出了所提出方法的可靠性。此外，通过流式细胞术和PARR定义的淋巴瘤亚型显示出显著的生存差异，证实了这些方法在预后方面的实用性。所提出的方法与细胞学评估具有高度一致性，并为猫淋巴增殖性疾病的特征描述和管理提供了一种额外的工具。
