Department of Laboratory Medicine, School of Medicine, Jiangsu University, Zhenjiang, 212013, Jiangsu, China.
Health Commission of Qinghai Province, Xining, 810008, Qinghai, China.
Curr Microbiol. 2024 Aug 28;81(10):330. doi: 10.1007/s00284-024-03855-x.
The type VI secretion system 2 (T6SS2) gene cluster of Vibrio parahaemolyticus comprises three operons: VPA1027-1024, VPA1043-1028, and VPA1044-1046. AcsS is a LysR-like transcriptional regulator that play a role in activating flagella-driven motility in V. parahaemolyticus. However, its potential roles in other cellular pathways remain poorly understood. In this study, we conducted a series of experiments to investigate the regulatory effects of AcsS on the transcription of VPA1027 (hcp2), VPA1043, and VPA1044. The findings revealed that AcsS indirectly inhibits the transcription of these genes. Additionally, deletion of acsS resulted in enhanced adhesion of V. parahaemolyticus to HeLa cells. However, disruption of T6SS2 alone or in conjunction with AcsS significantly diminished the adhesion capacity of V. parahaemolyticus to HeLa cells. Therefore, it is suggested that AcsS suppresses cell adhesion in V. parahaemolyticus by downregulating the transcription of T6SS2 genes.
副溶血弧菌的 6 型分泌系统 2（T6SS2）基因簇由三个操纵子组成：VPA1027-1024、VPA1043-1028 和 VPA1044-1046。AcsS 是一种 LysR 样转录调节因子，在副溶血弧菌中发挥激活鞭毛驱动运动的作用。然而，其在其他细胞途径中的潜在作用仍知之甚少。在这项研究中，我们进行了一系列实验来研究 AcsS 对 VPA1027（hcp2）、VPA1043 和 VPA1044 转录的调控作用。研究结果表明，AcsS 间接抑制这些基因的转录。此外，acsS 的缺失导致副溶血弧菌对 HeLa 细胞的黏附增强。然而，单独或与 AcsS 一起破坏 T6SS2 显著降低了副溶血弧菌对 HeLa 细胞的黏附能力。因此，推测 AcsS 通过下调 T6SS2 基因的转录来抑制副溶血弧菌的细胞黏附。
