Institute of Bioengineering, Federal Research Center "Fundamentals of Biotechnology" of the Russian Academy of Sciences, 119071, Moscow, Russia.
Dokl Biochem Biophys. 2024 Oct;518(1):393-397. doi: 10.1134/S1607672924600611. Epub 2024 Aug 28.
The circadian dynamics of the expression of key genes of carotenoid metabolism (PSY2, LCYE, CrtRB1, and NCED1) in the photosynthetic tissue of tomato Solanum lycopersicum L. (cultivar Korneevsky) plants was characterized. An in silico analysis of the gene expression pattern was carried out and a high level of their transcripts was detected in the leaf tissue. qRT-PCR analysis of gene expression was performed at six time points during the day and showed the highest levels of PSY2, LCYE, and NCED1 transcripts in the second half of the light phase and CrtRB1 at the end of the dark phase. The content and composition of carotenoids in leaf tissue in the middle of the day was determined; it was shown that the leaf accumulates 1.5 times more compounds of the ɛ/β-branch of carotenoid biosynthesis pathway than compounds of the β/β-branch.
对番茄（Solanum lycopersicum L.，品种 Korneevsky）植物光合作用组织中类胡萝卜素代谢关键基因（PSY2、LCYE、CrtRB1 和 NCED1）表达的昼夜动态进行了研究。对基因表达模式进行了计算机分析，发现叶片组织中这些基因的转录本水平较高。在一天中的六个时间点进行 qRT-PCR 分析基因表达，结果表明 PSY2、LCYE 和 NCED1 的转录本在光照阶段的后半段达到最高水平，而 CrtRB1 在暗期结束时达到最高水平。在一天的中午测定了叶片组织中类胡萝卜素的含量和组成，结果表明，与β/β-分支的化合物相比，叶片组织积累了 1.5 倍更多的ε/β-分支的类胡萝卜素生物合成途径的化合物。
