Integrated Devices and Intelligent Diagnosis (ID2) Laboratory, CUHK(SZ)-Boyalife Joint Laboratory for Regenerative Medicine Engineering, Biomedical Engineering Programme, School of Medicine, The Chinese University of Hong Kong, Shenzhen, 518172, China.
J Mater Chem B. 2024 Oct 2;12(38):9703-9712. doi: 10.1039/d4tb01459f.
Eutectic gallium indium (EGaIn) nanoparticles can be modified with aniline derivatives to introduce versatile functional groups toward bioapplications beyond materials science. -Aminobenzoic acid (PABA) modified EGaIn (EGaIn-PABA) demonstrated high wettability due to the presence of carboxyl groups, but the priority of binding of carboxyl and amino groups on the EGaIn surface remains unclear. To study the binding nature of PABA to EGaIn, the anti-mouse IgG antibody labeled with fluorescein isothiocyanate (FITC) (IgG-FITC) was covalently modified to EGaIn-PABA to verify the presence of terminal carboxyl groups on the EGaIn surface. The binding of gold nanoparticles (AuNPs) to EGaIn-PABA nanoparticles suggested the presence of terminal amino groups on the EGaIn surface. Then, taking advantage of the reductive nature of amino groups, the Almar blue fluorescence experiment was designed to determine the co-existence of carboxyl and amino groups on the EGaIn-PABA surface with an approximate ratio of 3 : 7, suggesting that carboxyl groups had a higher probability of binding with the EGaIn surface than that of amino groups. Then, an aptasensor was fabricated on the EGaIn-PABA surface with AuNPs for electrochemical detection of interleukin-6 with a sensitivity of 1 pg mL.
共晶镓铟（EGaIn）纳米粒子可以用苯胺衍生物进行修饰，从而在超越材料科学的生物应用中引入多功能基团。-氨基苯甲酸（PABA）修饰的 EGaIn（EGaIn-PABA）由于存在羧基而表现出高润湿性，但 EGaIn 表面上羧基和氨基的优先结合性质尚不清楚。为了研究 PABA 与 EGaIn 的结合性质，用异硫氰酸荧光素（FITC）标记的抗小鼠 IgG 抗体（IgG-FITC）对 EGaIn-PABA 进行了共价修饰，以验证 EGaIn 表面上末端羧基的存在。金纳米粒子（AuNPs）与 EGaIn-PABA 纳米粒子的结合表明 EGaIn 表面上存在末端氨基。然后，利用氨基的还原性质，设计了 Almar 蓝荧光实验来确定 EGaIn-PABA 表面上羧基和氨基的共存，其近似比例为 3:7，表明羧基与 EGaIn 表面的结合概率高于氨基。然后，在 EGaIn-PABA 表面上制备了带有 AuNPs 的适体传感器，用于电化学检测白细胞介素-6，其灵敏度为 1 pg mL-1。
