Mukhopadhyay N K, Ghosh S K, Majumder S, Bose S K
Biochem J. 1985 Feb 1;225(3):639-43. doi: 10.1042/bj2250639.
The extracellular release of mycobacillin from Bacillus subtilis first occurred in the medium at the onset of stationary phase and continued at a high rate even after 6 days. Mycobacillin synthetase activity appeared earlier than late-exponential phase in the cytosol of producer cells and was not sedimentable even at 105 000 g. The activity then quickly reached the maximum late in the stationary phase. With further increase in the age of the culture, the activity gradually disappeared from the cytosol, to reappear concomitantly in the membrane in an insoluble particulate form, even in absence of protein synthesis. The membrane-bound synthetase activity was sedimentable at 10 000 g and was fairly active even after 5 days.
枯草芽孢杆菌产生的分枝杆菌素在稳定期开始时首先在培养基中发生胞外释放，甚至在6天后仍以高速率持续释放。分枝杆菌素合成酶活性在产生菌细胞的胞质溶胶中比指数后期出现得更早，即使在105 000 g的离心力下也不可沉降。然后该活性在稳定期末期迅速达到最大值。随着培养时间的进一步延长，该活性逐渐从胞质溶胶中消失，即使在没有蛋白质合成的情况下，也会以不溶性颗粒形式同时重新出现在细胞膜中。膜结合的合成酶活性在10 000 g的离心力下可沉降，即使在5天后仍相当活跃。
