Department for Life Quality Studies, Alma Mater Studiorum - University of Bologna, Corso d'Augusto 237, Rimini 47921, Italy.
Research group of Pharmaco-Toxicological Analysis (PTA Lab), Department of Pharmacy and Biotechnology (FaBiT), Alma Mater Studiorum - University of Bologna, Via Belmeloro 6, Bologna 40126, Italy.
J Pharm Biomed Anal. 2024 Dec 15;251:116422. doi: 10.1016/j.jpba.2024.116422. Epub 2024 Aug 14.
Cannabis sativa L. is a plant belonging to the Cannabaceae family known primarily for its recreational use due to the psychoactive properties of Δ-tetrahydrocannabinol (THC). Despite this, several compounds belonging to the category of phytocannabinoids have shown in recent years a number of potentially promising therapeutic effects that have increased the interest in the pharmaceutical field towards this plant. However, the content of these compounds is very variable and influenced by different factors, such as growing conditions and time of the year. An indication of the status and age of Cannabis samples is provided by the content of CBN, a minor phytocannabinoid and degradation product of other phytocannabinoids, including THC. In this research work an innovative, solid state analytical approach has been developed to observe and evaluate the variations in the content of two phytocannabinoids (CBN and CBD) in Cannabis-derived products over time. In order to simulate the ageing of the Cannabis samples, an artificially accelerated ageing procedure has been developed and optimised by using high temperatures. The analyses were carried out using an innovative ATR-FTIR method for solid state analysis, enabling direct analysis of a solid sample without any pretreatment phase. This study has allowed the development of an innovative analytical approach for the evaluation of the age and state of conservation of Cannabis samples and may be a useful tool both in the industrial, pharmaceutical and forensic fields.
大麻（Cannabis sativa L.）是大麻科植物，主要因其精神活性成分 Δ-四氢大麻酚（THC）而被用于娱乐用途。尽管如此，近年来属于植物大麻素类别的几种化合物表现出了许多有希望的潜在治疗效果，这增加了人们对该植物在制药领域的兴趣。然而，这些化合物的含量变化很大，受到不同因素的影响，如生长条件和一年中的时间。CBN 的含量可作为大麻样品状况和年龄的指示物，CBN 是一种次要的植物大麻素，也是其他植物大麻素（包括 THC）的降解产物。在这项研究工作中，开发了一种创新的固态分析方法，以观察和评估随时间推移大麻衍生产品中两种植物大麻素（CBN 和 CBD）含量的变化。为了模拟大麻样品的老化，通过使用高温开发并优化了一种人为加速老化程序。分析使用创新的 ATR-FTIR 方法进行固态分析，可直接对无任何预处理阶段的固体样品进行分析。这项研究开发了一种用于评估大麻样品的年龄和保存状态的创新分析方法，可能是工业、制药和法医学领域的有用工具。
