National Center for Natural Products Research, School of Pharmacy, The University of Mississippi, University, Mississippi, United States of America.
Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Suez Canal University, Ismailia, Egypt.
Planta Med. 2018 Mar;84(4):250-259. doi: 10.1055/s-0043-124088. Epub 2017 Dec 13.
Cannabis ( L.) is an annual herbaceous plant that belongs to the family Cannabaceae. -Δ-tetrahydrocannabinol (Δ-THC) and cannabidiol (CBD) are the two major phytocannabinoids accounting for over 40% of the cannabis plant extracts, depending on the variety. At the University of Mississippi, different strains of with different concentration ratios of CBD and Δ-THC, have been tissue cultured via micropropagation and cultivated. A GC-FID method has been developed and validated for the qualitative and quantitative analysis of acid and neutral cannabinoids in extracts. The method involves trimethyl silyl derivatization of the extracts. These cannabinoids include tetrahydrocannabivarian, CBD, cannabichromene, -Δ-tetrahydrocannabinol, Δ-THC, cannabigerol, cannabinol, cannabidiolic acid, cannabigerolic acid, and Δ-tetrahydrocannabinolic acid-A. The concentration-response relationship of the method indicated a linear relationship between the concentration and peak area ratio with R > 0.999 for all 10 cannabinoids. The precision and accuracy of the method were found to be ≤ 15% and ± 5%, respectively. The limit of detection range was 0.11 - 0.19 µg/mL, and the limit of quantitation was 0.34 - 0.56 µg/mL for all 10 cannabinoids. The developed method is simple, sensitive, reproducible, and suitable for the detection and quantitation of acidic and neutral cannabinoids in different extracts of cannabis varieties. The method was applied to the analysis of these cannabinoids in different parts of the micropropagated cannabis plants (buds, leaves, roots, and stems).
大麻（L.）是一种一年生草本植物，属于大麻科。-Δ-四氢大麻酚（Δ-THC）和大麻二酚（CBD）是两种主要的植物大麻素，占大麻植物提取物的 40%以上，具体取决于品种。在密西西比大学，通过微繁殖培养了具有不同 CBD 和 Δ-THC 浓度比的不同品种的大麻，并对其进行了组织培养。已经开发并验证了一种 GC-FID 方法，用于定性和定量分析大麻提取物中的酸性和中性大麻素。该方法涉及提取物的三甲基硅烷基衍生化。这些大麻素包括四氢大麻酚、CBD、大麻色烯、-Δ-四氢大麻酚、Δ-THC、大麻二醇、大麻醇、大麻酸、大麻酸和 Δ-四氢大麻酚酸-A。方法的浓度-响应关系表明，所有 10 种大麻素的浓度与峰面积比之间呈线性关系，R > 0.999。该方法的精密度和准确度分别为≤15%和±5%。检测限范围为 0.11-0.19 µg/mL，定量限为 0.34-0.56 µg/mL，所有 10 种大麻素均适用。所开发的方法简单、灵敏、重现性好，适用于不同大麻品种提取物中酸性和中性大麻素的检测和定量。该方法应用于微繁殖大麻植物（芽、叶、根和茎）不同部位中这些大麻素的分析。
