Zhang Tong, Liang Xiaojing, Si Tiantian, Lu Xiaofeng, Wang Shuai
Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China; University of Chinese Academy of Sciences, Beijing 100049, China.
Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China.
Talanta. 2024 Dec 1;280:126768. doi: 10.1016/j.talanta.2024.126768. Epub 2024 Aug 24.
In response to the challenges associated with the chromatographic separation of polar compounds, this study aims to devise a solution by introducing a novel stationary phase. Hydrogels, characterized by a three-dimensional network structure, have aroused wide attention owing to its functional designability, multiple interaction sites and good adhesion, etc. In this work, an adhesive hydrogel functionalized silica stationary phase (Sil@PVA/TA) was synthesized using physical coating technique. Due to the co-existence of hydroxyl and benzene ring in the hydrogel structure, the obtained composites materials exhibited excellent separation performance for various of compounds and excellent column efficiency up to 71385.6 plates/m for thymidine. Furthermore, the hydrogel functionalized silica demonstrated superior selectivity to bare silica, diol-column and NH-column for the separation of various of polar molecules, including, nucleosides/bases, alkaloids, organic acids, antibiotics and amino acids. Notably, for alkaloids, which frequently encounter peak tailing issues, Sil@PVA/TA demonstrated superior peak shape compared with C18 column. In short, this study successfully synthesized a hydrogel functionalized silica stationary phase, offering a novel method for the separation and analysis of polar compounds.
针对极性化合物色谱分离相关的挑战，本研究旨在通过引入一种新型固定相来设计解决方案。水凝胶具有三维网络结构，因其功能可设计性、多个相互作用位点和良好的粘附性等特点而引起了广泛关注。在这项工作中，采用物理涂覆技术合成了一种粘合剂水凝胶功能化硅胶固定相（Sil@PVA/TA）。由于水凝胶结构中羟基和苯环的共存，所得复合材料对各种化合物表现出优异的分离性能，对胸腺嘧啶核苷的柱效高达71385.6塔板/米。此外，水凝胶功能化硅胶在分离各种极性分子（包括核苷/碱基、生物碱、有机酸、抗生素和氨基酸）方面对裸硅胶、二醇柱和NH柱表现出卓越的选择性。值得注意的是，对于经常出现峰拖尾问题的生物碱，Sil@PVA/TA与C18柱相比表现出更优的峰形。简而言之，本研究成功合成了一种水凝胶功能化硅胶固定相，为极性化合物的分离分析提供了一种新方法。
