Division of Natural Sciences, Northeast Texas Community College, 2886 FM 1735, Chapel Hill Road, Mt. Pleasant, Texas 75455.
P.O. Box 197, Burdett, Kansas 67523.
J Parasitol. 2024 Jul 1;110(4):414-422. doi: 10.1645/23-65.
During March 2023, 7 green sunfish (Lepomis cyanellus) and 2 bluegill (Lepomis macrochirus) were collected from the Black River (White River drainage) in Lawrence County, Arkansas. In addition, during March 2023 and again in May-June 2023, 13 L. cyanellus and 6 L. macrochirus were taken from Butcherknife and Big Fork creeks (Ouachita River drainage), Polk County, Arkansas, 9 L. cyanellus were collected from the Caddo River, Montgomery County, Arkansas, and 5 green sunfish were taken from Clear Creek at Savoy, Washington County, Arkansas. All fish had their gill, gallbladder, fins, integument, musculature, and other major organs examined for myxozoans. The gill of 1 of 34 (3%) L. cyanellus was infected with a new myxozoan, Myxobolus fergusoni n. sp. Qualitative and quantitative morphological data were obtained from fresh myxospores, and molecular data consisted of a 1,933-base-pair sequence of the partial small subunit (SSU) ribosomal RNA (rRNA) gene. Phylogenetic analysis grouped M. fergusoni n. sp. with other centrarchid-infecting myxobolids from North America and placed this cluster in a larger clade comprising myxozoans that infect North American and European esocids, a North American aphredoderid, European percids, and a gasterosteid from Japan. Myxobolus fergusoni n. sp. infects the gill arches of L. cyanellus, similar to Myxobolus cartilaginis (Hoffman, Putz, and Dunbar, 1965), which was described from head cartilage, gill arches, and large fin rays of L. cyanellus. Another is Myxobolus mesentericusKudo, 1920, which was described from the viscera of green sunfish. A large polysporic plasmodium filled with myxospores was present in a basifilamental location associated with multiple gill filaments at their junction with the gill arch. The intact plasmodium replaced connective tissue within the arch but elicited only mild proliferation of overlying epithelium and a minimal host inflammatory response. This is the third time a myxozoan has been described from L. cyanellus, as well as being the first time it has been described from an Arkansas specimen.
在 2023 年 3 月期间，从阿肯色州劳伦斯县的黑河（怀特河流域）采集了 7 条绿太阳鱼（Lepomis cyanellus）和 2 条蓝鳃太阳鱼（Lepomis macrochirus）。此外，在 2023 年 3 月和 2023 年 5 月至 6 月期间，从阿肯色州波克县的 Butcherknife 和 Big Fork 溪流（欧扎克斯河流域）采集了 13 条绿太阳鱼和 6 条蓝鳃太阳鱼，从阿肯色州蒙哥马利县的 Caddo 河采集了 9 条绿太阳鱼，从阿肯色州华盛顿县的 Savoy 的 Clear Creek 采集了 5 条绿太阳鱼。对所有鱼类的鳃、胆囊、鳍、表皮、肌肉和其他主要器官进行了检查，以寻找粘孢子虫。在 34 条绿太阳鱼中的 1 条（3%）的鳃中发现了一种新的粘孢子虫，Myxobolus fergusoni n. sp.。从新鲜的孢子中获得了定性和定量形态学数据，分子数据由部分小亚基（SSU）核糖体 RNA（rRNA）基因的 1933 个碱基对序列组成。系统发育分析将 M. fergusoni n. sp.与来自北美的其他感染鲈形目粘孢子虫聚类在一起，并将该聚类置于一个更大的分支中，其中包括感染北美的和欧洲的梭鲈、北美的拟鲤科、欧洲的鲈科和来自日本的胡瓜鱼科鱼类的粘孢子虫。Myxobolus fergusoni n. sp.感染绿太阳鱼的鳃弓，类似于 Myxobolus cartilaginis（Hoffman、Putz 和 Dunbar，1965），后者从绿太阳鱼的头部软骨、鳃弓和大鳍射线中描述。另一种是 Myxobolus mesentericusKudo，1920 年，从绿太阳鱼的内脏中描述。一个充满孢子的大多孢子质团位于与鳃弓相连的多个鳃丝的基膜位置。完整的质团取代了弓内的结缔组织，但仅引起覆盖上皮的轻度增殖和最小的宿主炎症反应。这是第三次从绿太阳鱼中描述粘孢子虫，也是第一次从阿肯色州的标本中描述。
