Department of Cardiovascular Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1277 Jiefang Avenue, Wuhan, China.
Hubei Engineering Research Center for Biomaterials and Medical Protective Materials, Hubei Key Laboratory of Bioinorganic Chemistry & Materia Medica, State Key Laboratory of Materials Processing and Die & Mould Technology, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology (HUST), 1037 Luoyu Road, Wuhan, China.
Nat Commun. 2024 Aug 29;15(1):7462. doi: 10.1038/s41467-024-51773-0.
Regenerative heart valve prostheses are essential for treating valvular heart disease, which requested interactive materials that can adapt to the tissue remodeling process. Such materials typically involves intricate designs with multiple active components, limiting their translational potential. This study introduces a facile method to engineer interactive materials for heart valve regeneration using 1,1'-thiocarbonyldiimidazole (TCDI) chemistry. TCDI crosslinking forms cleavable thiourea and thiocarbamate linkages which could gradually release HS during degradation, therefore regulates the immune microenvironment and accelerates tissue remodeling. By employing this approach, a double network hydrogel was formed on decellularized heart valves (DHVs), showcasing robust anti-calcification and anti-thrombosis properties post fatigue testing. Post-implantation, the DHVs could adaptively degrade during recellularization, releasing HS to further support tissue regeneration. Therefore, the comprehensive endothelial cell coverage and notable extracellular matrix remodeling could be clearly observed. This accessible and integrated strategy effectively overcomes various limitations of bioprosthetic valves, showing promise as an attractive approach for immune modulation of biomaterials.
再生心脏瓣膜假体对于治疗心脏瓣膜疾病至关重要，这就需要能够适应组织重塑过程的交互材料。这些材料通常涉及具有多个活性成分的复杂设计，限制了它们的转化潜力。本研究介绍了一种使用 1,1'-硫代羰基二咪唑 (TCDI) 化学工程用于心脏瓣膜再生的交互式材料的简便方法。TCDI 交联形成可裂解的硫脲和硫代氨基甲酸酯键，在降解过程中可逐渐释放 HS，从而调节免疫微环境并加速组织重塑。通过采用这种方法，在去细胞心脏瓣膜 (DHV) 上形成了双网络水凝胶，在疲劳试验后表现出强大的抗钙化和抗血栓形成特性。植入后，DHV 在再细胞化过程中能够自适应降解，释放 HS 以进一步支持组织再生。因此，可以清楚地观察到全面的内皮细胞覆盖和显著的细胞外基质重塑。这种易于使用且集成的策略有效地克服了生物假体的各种限制，有望成为生物材料免疫调节的一种有吸引力的方法。
