mTOR 基因变异 rs2295080 可能是伊朗 2 型糖尿病女性发生动脉粥样硬化的危险因素。
mTOR gene variant rs2295080 might be a risk factor for atherosclerosis in Iranian women with type 2 diabetes mellitus.
机构信息
Department of Immunology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
Cardiovascular Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
出版信息
BMC Endocr Disord. 2024 Aug 29;24(1):162. doi: 10.1186/s12902-024-01703-4.
BACKGROUND
Type 2 diabetes mellitus, one of the most prevalent metabolic disorders worldwide, is closely linked with an enhanced risk of atherosclerosis. However, the molecular mechanism of this linkage is not still clear. Genetic variations in the mTOR gene may increase the susceptibility of individuals to these diseases.
METHODS
One hundred nine diabetic patients and 375 healthy subjects participated in this study. mTOR Single Nucleotide Polymorphism (SNP) rs2295080 was determined using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP).
RESULTS
Comparison of genotypic, allelic, and genotypic combination frequencies between cases and controls revealed no significant result. Nevertheless, the frequency of rs2295080 GT + TT genotype was significantly more in diabetic women with atherosclerosis compared with those without atherosclerosis (p = 0.047). Besides, the rs2295080 G allele was more frequently detected in diabetic women without atherosclerosis compared to those with atherosclerosis (p = 0.046).
CONCLUSION
The rs2295080 GT + TT genotype predisposes Iranian diabetic women to atherosclerosis, while the rs2295080 G allele protects them against atherosclerosis. However, additional experiments using larger sample sizes are needed to verify this result.
背景
2 型糖尿病是全球最常见的代谢紊乱之一，与动脉粥样硬化风险增加密切相关。然而，这种联系的分子机制尚不清楚。mTOR 基因的遗传变异可能会增加个体患这些疾病的易感性。
方法
109 例糖尿病患者和 375 例健康受试者参与了这项研究。使用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）法确定 mTOR 单核苷酸多态性（SNP）rs2295080。
结果
病例组和对照组之间基因型、等位基因和基因型组合频率的比较没有显著结果。然而，与无动脉粥样硬化的糖尿病女性相比，有动脉粥样硬化的糖尿病女性 rs2295080 GT+TT 基因型的频率显著更高（p=0.047）。此外，与有动脉粥样硬化的糖尿病女性相比，无动脉粥样硬化的糖尿病女性 rs2295080 G 等位基因的频率更高（p=0.046）。
结论
rs2295080 GT+TT 基因型使伊朗糖尿病女性易患动脉粥样硬化，而 rs2295080 G 等位基因则使她们免受动脉粥样硬化的影响。然而，需要使用更大的样本量进行额外的实验来验证这一结果。
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