Wang Xiao, Chen Bingjie, Bhullar Khushwant S, Yang Hang, Luo Xiaohu, Fu Juan, Liu Hongru, Su Di, Sun Dapeng, Qiao Yongjin, Zhou Wenzong
Crop Breeding and Cultivation Research Institution, Research Center for Agricultural Products Preservation and Processing, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China.
Department of Agricultural Food & Nutritional Science, University of Alberta, Edmonton, AB T6G 2P5, Canada.
Antioxidants (Basel). 2024 Jul 23;13(8):888. doi: 10.3390/antiox13080888.
Sixteen novel antioxidant peptides from Asian swamp eel (ASE) were identified in previous studies. However, their chemical and cellular antioxidant mechanisms remain unclear. Molecular docking of these peptides with ABTS and DPPH radicals revealed the critical role of hydrogen bonding and Pi-Pi stacking hydrophobic interactions between hydrophobic amino acid residues and free radicals. Residues, such as tryptophan, proline, leucine, and valine, played significant roles in these interactions. All these peptides exhibited notable erythrocyte morphoprotective effects in a model of AAPH-induced oxidative damage of human erythrocytes. Erythrocyte hemolysis was reduced primarily through the modulation of both non-enzymatic (GSH/GSSG) and enzymatic antioxidant systems (SOD, CAT, and GSH-Px) by these peptides. A decrease in levels of MDA, LDH release, and hemoglobin oxidation was observed. Among the peptides, VLYPW demonstrated superior chemical and cellular antioxidant activities, which may be attributed to its higher levels of tyrosine and tryptophan, as well as to its increased hydrophobic amino acid content.
先前的研究中从黄鳝中鉴定出了16种新型抗氧化肽。然而，它们的化学和细胞抗氧化机制仍不清楚。这些肽与ABTS和DPPH自由基的分子对接揭示了疏水氨基酸残基与自由基之间氢键和π-π堆积疏水相互作用的关键作用。色氨酸、脯氨酸、亮氨酸和缬氨酸等残基在这些相互作用中发挥了重要作用。在AAPH诱导的人红细胞氧化损伤模型中，所有这些肽都表现出显著的红细胞形态保护作用。这些肽主要通过调节非酶（GSH/GSSG）和酶抗氧化系统（SOD、CAT和GSH-Px）来减少红细胞溶血。观察到丙二醛水平降低、乳酸脱氢酶释放减少和血红蛋白氧化减少。在这些肽中，VLYPW表现出卓越的化学和细胞抗氧化活性，这可能归因于其较高的酪氨酸和色氨酸水平，以及其增加的疏水氨基酸含量。
