Ji Qiurong, Zhang Fengshuo, Zhang Yu, Su Quyangangmao, He Tingli, Hou Shengzhen, Gui Linsheng
College of Agriculture and Animal Husbandry, Qinghai University, Xining 810016, China.
Antioxidants (Basel). 2024 Jul 24;13(8):892. doi: 10.3390/antiox13080892.
Previous research studies confirmed that both resveratrol (RES) and β-hydroxy-β-methyl butyric acid (HMB) improved growth performance by altering intestinal microbiota. However, the mechanism underlying of RES and HMB on intestinal function remains unclear in ruminant. In this study, supplements of RES and HMB alone or in combination were evaluated as promoters of antioxidant capacity, immune response and barrier function, and modulators of the microbiota and metabolite profiles in the jejunum of Tibetan sheep. A total of 120 two-month-old Tibetan rams were randomly divided into four treatments ( = 30 per treatment), which were supplemented with a basal diet with 1.5 g RES/d (RES group), 1.25 g HMB/d (HMB group), 1.5 g RES/d plus 1.25 g HMB/d (RES-HMB group), and without additions (Control group). The results showed that RES and HMB improved the antioxidant capacity (CAT, GSH-Px, SOD, and T-AOC), immunity (IgA, IgG, and IgM), and digestive enzyme activity (α-amylase, lipase, and chymotrypsin) of the experimental lambs ( < 0.05). Additionally, jejunal morphology including villus width, villus height, and muscle layer thickness exhibited a significant difference when rams were fed diets supplemented with RES and HMB ( < 0.05). Furthermore, the determination of fermentation parameters showed that the butyrate concentration in the RES-HMB group was greater than those in the C and RES groups ( < 0.05). When compared to the C group, barrier-related gene expression (, , and ) was significantly increased in the RES-HMB group ( < 0.05). Dietary RES and (or) HMB supplementation significantly increased the abundance of , and ( < 0.05). The abundance of differential bacteria was positively associated with butyrate concentration ( < 0.05). Metabolome analysis revealed that alpha ketoglutarate, succinic semialdehyde, and diacetyl as well as butanoate metabolism pathways connected to the improvements in butyrate concentration by RES and (or) HMB supplementation. Collectively, our results suggested that RES and (or) HMB supplementation improved butyrate concentration via regulating the microbial community (, and ) and metabolism (alpha ketoglutarate, succinic semialdehyde, and diacetyl), thus contributing to jejunal morphology, antioxidant capacity, immune response, digestive enzyme activity, and barrier function.
先前的研究证实，白藜芦醇（RES）和β-羟基-β-甲基丁酸（HMB）均通过改变肠道微生物群来改善生长性能。然而，RES和HMB对反刍动物肠道功能的潜在作用机制仍不清楚。在本研究中，单独或联合添加RES和HMB被评估为藏羊空肠抗氧化能力、免疫反应和屏障功能的促进剂，以及微生物群和代谢物谱的调节剂。总共120只两个月大的藏羊公羊被随机分为四个处理组（每组n = 30），分别添加基础日粮，其中一组添加1.5 g/d RES（RES组），一组添加1.25 g/d HMB（HMB组），一组添加1.5 g/d RES加1.25 g/d HMB（RES-HMB组），还有一组不添加（对照组）。结果表明，RES和HMB提高了试验羔羊的抗氧化能力（CAT、GSH-Px、SOD和T-AOC）、免疫力（IgA、IgG和IgM）和消化酶活性（α-淀粉酶、脂肪酶和胰凝乳蛋白酶）（P < 0.05）。此外，当公羊饲喂添加RES和HMB的日粮时，空肠形态包括绒毛宽度、绒毛高度和肌层厚度表现出显著差异（P < 0.05）。此外，发酵参数的测定表明，RES-HMB组的丁酸盐浓度高于对照组和RES组（P < 0.05）。与对照组相比，RES-HMB组中与屏障相关的基因表达（Occludin、Claudin-1和ZO-1）显著增加（P < 0.05）。日粮中添加RES和（或）HMB显著增加了Ruminococcus、Butyrivibrio和Faecalibacterium的丰度（P < 0.05）。差异细菌的丰度与丁酸盐浓度呈正相关（P < 0.05）。代谢组分析表明，α-酮戊二酸、琥珀半醛、双乙酰以及丁酸代谢途径与RES和（或）HMB添加导致的丁酸盐浓度升高有关。总的来说，我们的结果表明，添加RES和（或）HMB通过调节微生物群落（Ruminococcus、Butyrivibrio和Faecalibacterium）和代谢（α-酮戊二酸、琥珀半醛和双乙酰）提高了丁酸盐浓度，从而有助于空肠形态、抗氧化能力、免疫反应、消化酶活性和屏障功能。
