Chong Joyce Ruifen, de Lucia Chiara, Tovar-Rios Diego Alejandro, Castellanos-Perilla Nicolas, Collins Christopher, Kvernberg Silje Meihack, Ballard Clive, Siow Richard C, Aarsland Dag
Centre for Healthy Brain Ageing, Institute of Psychiatry, Psychology and Neuroscience, King's College London, London S5 9NU, UK.
Department of Pharmacology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 117600, Singapore.
Antioxidants (Basel). 2024 Aug 17;13(8):995. doi: 10.3390/antiox13080995.
DailyColors™ is a supplement made up of several phytonutrients that aims to replicate elements from the Mediterranean diet. These include fruit, berry and vegetable extracts that are rich in key phytochemicals such as Quercetin, Catechins, Phloretin, Ellagic Acid, and Anthocyanins. Here, we determined the effects of DailyColors™ on the blood biomarkers associated with the diverse mechanisms implicated in ageing and age-related diseases, including mitochondrial function, inflammation, and oxidative stress, as well as on saliva's DNA methylation pattern. Thirty adult participants (mean (SD) age = 67.0 (7.5) years) with a body mass index over 25 were recruited into this randomised, double-blind, placebo-controlled, cross-over trial (two one-week treatment periods, separated by a one-week washout period). During the placebo period, we observed a significant increase in blood CD38 concentrations from the baseline to 24 h (-value = 0.019). This was not observed in the active period. Increased CD38 is reportedly associated with subsequent mitochondrial dysfunction and inflammation. Next, there was a decreasing trend of plasma 4-HNE levels, an oxidative stress biomarker, after a one-week intake of DailyColors™. Furthermore, following a one-month open-label follow-up in 26 participants, we observed hypermethylation of the candidate CpG site cg13108341 (q-value = 0.021), which was against the observed trend for this site during ageing. Taken together, while minimal effects were observed in this study, DailyColors™ supplementation may be beneficial by altering and alleviating age-related changes. Longer and larger scale trials of DailyColors™ supplementation are warranted.
DailyColors™是一种由多种植物营养素组成的补充剂，旨在复制地中海饮食中的成分。这些成分包括富含关键植物化学物质（如槲皮素、儿茶素、根皮素、鞣花酸和花青素）的水果、浆果和蔬菜提取物。在此，我们确定了DailyColors™对与衰老和年龄相关疾病所涉及的多种机制相关的血液生物标志物的影响，包括线粒体功能、炎症和氧化应激，以及对唾液DNA甲基化模式的影响。30名体重指数超过25的成年参与者（平均（标准差）年龄 = 67.0（7.5）岁）被招募到这项随机、双盲、安慰剂对照、交叉试验中（两个为期一周的治疗期，中间有一周的洗脱期）。在安慰剂期，我们观察到血液中CD38浓度从基线到24小时显著增加（P值 = 0.019）。在活性期未观察到这种情况。据报道，CD38增加与随后的线粒体功能障碍和炎症有关。其次，在摄入DailyColors™一周后，氧化应激生物标志物血浆4-HNE水平有下降趋势。此外，在26名参与者进行为期一个月的开放标签随访后，我们观察到候选CpG位点cg13108341发生了高甲基化（q值 = 0.021），这与该位点在衰老过程中观察到的趋势相反。综上所述，虽然本研究中观察到的影响最小，但补充DailyColors™可能通过改变和减轻与年龄相关的变化而有益。有必要对DailyColors™补充剂进行更长时间和更大规模的试验。
